FastQCFastQC Report
Sun 2 Jul 2023
SRR3590744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127657
Total Bases6.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1276810.00180170300101Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA45843.5908724159270546No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG32172.520034154022106No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG23211.8181533327588772No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG21041.648166571359189No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA9650.7559319112935444No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7870.6164957659979476No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG7850.6149290677361994No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA6530.511526982460813No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5120.4010747550075593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA4970.38932451804444723Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3970.3109896049570333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3720.29140587668517987No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3590.281222337983816Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3530.27652224319857116No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG2780.21777105838301072No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2320.18173699836280033Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2220.17390350705405894No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2220.17390350705405894No Hit
ATAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2180.17077011053056237No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGGCTGAACTCCAGTCA2050.16058657182919855Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2030.1590198735674503No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1960.1535364296513313No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1930.15118638225870887No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1880.1472696366043382No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGGCA1870.14648628747346407Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGAGGGGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1700.13316935224860368No Hit
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC1650.129252606594233No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG1580.12376916267811403No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG1560.12220246441636573No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1520.11906906789286918No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1490.11671902050024675Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1480.11593567136937262Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1410.11045222745325364No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1400.10966887832237951Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1370.1073188309297571No Hit
ATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1340.10496878353713467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA1320.10340208527538639No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGCACTCCAGTCA1290.10105203788276396Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)