FastQCFastQC Report
Sun 2 Jul 2023
SRR3590745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301792
Total Bases15 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3269110.832295090658468Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA136304.5163556356695995No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG94553.132952497084084No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG74572.470907114834058No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG58011.9221848160322341No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA32171.06596596331248No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG26830.8890229031916022No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA26580.8807390520623477No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA22370.7412389990457003No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG15080.4996819001166366No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA14200.4705227441416605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG12710.42115099141130313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT12340.40889089174000637Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC10520.34858445551903294No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC10220.3386438341639275Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG9550.3164431131375252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG8760.29026614356908065No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG8040.2664086523168275No Hit
AGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAG7990.26475188209097655No Hit
ATAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6760.223995334535044No Hit
TGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGC6700.22200721026402293No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6670.22101314812851236Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA6180.2047767999151734No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA6120.20278867564415226No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA5980.19814971901176967No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5910.19583024069557842No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA5780.191522638108366No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5630.1865523274308133No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5000.16567702258509173No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4810.15938129572685825No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG4660.15441098504930548No Hit
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC4550.15076609055243345Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4530.1501033824620931No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4450.14745255010073163No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4300.1424822394231789Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAA4250.14082546919732794No Hit
GTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAG4080.13519245042943484No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA3980.13187890997773302No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3950.13088484784222246No Hit
ATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3900.12922807761637153No Hit
ATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3630.12028151839677659Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA3490.11564256176439401No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3450.1143171455837133Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3280.10868412681582017No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGAG3210.10636464849962889No Hit
ATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAA3150.10437652422860778No Hit
CGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3130.10371381613826741Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3060.10139433782207613No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3030.10040027568656558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)