FastQCFastQC Report
Sun 2 Jul 2023
SRR3590746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8965401
Total Bases448.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA739080.8243691498015537Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC433110.4830904942232924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT287150.3202868449498243No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC257290.2869810285117197No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC232610.2594529792922815No Hit
GCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA216640.24164005603318803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA213010.23759115738381364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC194390.21682242657076914No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG191000.21304122369986575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG183140.20427418695493932No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT139300.15537509142089684No Hit
ATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT133830.14927385846991115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT107210.11958193504116549Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC103350.11527649460408966No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC103230.11514264671485414No Hit
TCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA99540.11102682412086197Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC97930.1092310316069521No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC95150.10613022217299593No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA94340.1052267489206562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)