FastQCFastQC Report
Sun 2 Jul 2023
SRR3590747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12915322
Total Bases645.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1071790.8298592942553039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA552690.42793358152433214Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC529570.4100323631110398No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT423800.32813738596683845No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG394280.30528081297547205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA360290.27896323452098215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA305290.23637815611565857No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG216450.16759164037876872No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT209120.16191621083856833No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT207480.16064640122793686No Hit
ATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA190030.14713531726115694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC172210.13333775185783212No Hit
AAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT168850.13073619070434328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC168700.13062004958141965No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC166120.12862242226713358No Hit
GAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT152740.1182626341023476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC132650.10270746637211213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)