FastQCFastQC Report
Sun 2 Jul 2023
SRR3590748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287301
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG52621.8315286058872053No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG51121.7793185544080947No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA24060.8374492257249365Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC23710.8252668803798108No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA21810.7591341485062704No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA20700.7204987104117284No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA17260.6007636590196345Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG15590.5426364683728911Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG14570.5071336333670958No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA8370.291332087253438No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA8320.28959175220413436No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG8160.28402268004636255No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7930.2760171388195655No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA7420.25826572131666786No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7210.2509563141095924No Hit
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC5610.19526559253187423Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5160.179602577088141No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG5100.17751417502897657No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT5050.17577383997967289No Hit
TCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA4800.16707216473315442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA4500.15663015443733228No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC4400.15314948433872488No Hit
TCCTGGGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA4070.14166327301332055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3950.13748646889499166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3850.13400579879638427No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG3780.1315693297273591No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG3720.12948092766819466No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3680.12808865962875174Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3670.127740592618891Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG3620.1260002575695873No Hit
ATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3600.1253041235498658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTATTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3470.12077925242167623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG3390.11799471634279031No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3340.11625438129348661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3140.10929304109627185No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC3090.10755270604696818No Hit
TCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3040.10581237099766448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG3000.10442010295822152No Hit
ATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT2920.1016355668793356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)