FastQCFastQC Report
Sun 2 Jul 2023
SRR3590749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394491
Total Bases19.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG155773.948632541680292No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG153373.887794651842501No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA90862.3032211127757036No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC77101.9544172110390352No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA63631.6129645543244333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA45541.1543989596720836No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG42771.0841818951509667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA42501.0773376325442152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG39230.994446007640225No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA31510.798750795328664No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG29090.7374059230755581No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA24550.6223209147990701No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT24330.616744108230606No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA23700.6007741621481859No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT20310.5148406427523061No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA17400.4410747013239845No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT16790.4256117376568794No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC16580.42028842229607266No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA16070.40736037070554204No Hit
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC14730.3733925488794421Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG14470.3668017774803481No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAG14050.3561551467587347No Hit
TCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA13890.35209928743621527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT13630.34550851603712124No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG11790.2988661338281482No Hit
TCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA11640.29506376571328624Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG11590.2937963096749989No Hit
TCCTGGGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA11520.29202187122139667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG11180.283403170161043No Hit
TCCTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA11030.27960080204618104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC10930.2770658899696064No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG10700.27123559219348475No Hit
ATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA10500.26616576804033554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG10250.25982848784889895No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC10160.2575470669799818Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA9260.23473285829081017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC8380.21242563201695353No Hit
ATCTATTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA8320.21090468477100874Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG8300.2103977023556938No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC8190.2076092990714617Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT8000.20279296612596992Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG7990.20253947491831245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC7930.2010185276723677No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG7730.19594870351921845No Hit
TTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG7640.19366728265030128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA7540.19113237057372665No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC7540.19113237057372665No Hit
ATAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7270.18428810796697517No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7170.18175319589040057No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC7160.1814997046827431No Hit
CCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA7030.17820431898319608Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6800.17237402120707443No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA6630.16806467067689757No Hit
ATAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6400.16223437290077594No Hit
CCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA6230.15792502237059908Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA6200.15716454874762667No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT6030.15285519821744983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA5730.14525046198772595No Hit
TTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA5450.138152708173317No Hit
TAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA5380.13637826971971476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5340.1353643048890849No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC5310.13460383126611256No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5280.13384335764314015No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA5150.13054797194359313No Hit
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGA4930.12497116537512895No Hit
TCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4830.12243625329855434No Hit
TTGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC4440.11255009619991331No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA4430.11229660499225584No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA4420.11204311378459839No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAG4320.10950820170802376No Hit
TCAGGTCGAGGTCCTGATCCTGTAGGCACCATCAATAGATCGGAAGAGCA4200.1064663072161342No Hit
ATCGGGTGGTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4200.1064663072161342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4190.10621281600847676No Hit
TCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA4180.1059593248008193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGCTAGAGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4170.10570583359316182No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA4080.10342441272424466No Hit
TTGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAG4000.10139648306298496No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA3980.10088950064767004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)