FastQCFastQC Report
Sun 2 Jul 2023
SRR3590751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14241103
Total Bases712 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG711730.4997716820108667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA675040.47400822815479954Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC522020.36655868579842443No Hit
ACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT368890.25903190223397726No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG316600.22231424068767705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA237400.16670057087572498No Hit
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA234110.1643903565615669Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT206390.14492557212738366No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT193050.13555832016663316No Hit
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT192720.1353265965424167No Hit
AAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT163230.11461893085107243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG154710.10863624818948363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)