FastQCFastQC Report
Sun 2 Jul 2023
SRR3590752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9300499
Total Bases465 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4398094.728875300131746Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG564140.6065696044911139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA380480.40909632913244764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC338120.3635503858448885Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT260560.2801570109302738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA240620.25871730108244734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC207080.2226547199241675Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA194030.20862321473288692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC181980.19566692066737496No Hit
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG152440.16390518401216966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC149570.1608193280812137Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT131950.14187410804517048No Hit
TCCTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA128190.13783131421228043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC123940.13326166692776376Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA110030.11830548016832215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC103330.11110156562567233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)