FastQCFastQC Report
Sun 2 Jul 2023
SRR3590753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17682755
Total Bases884.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA11840496.696066308671924Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1529750.8651084064672049Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1055290.596790488812405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC935460.5290238992736144Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA731670.41377602076147074Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT701510.3967198550225913Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC629270.3558664925233653Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC597820.33808080245414246No Hit
CCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA552120.3122364133869411Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT478390.2705404220100318No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC461830.2611753654902757Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG411590.23276350319845523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG382410.21626154974154196Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC372090.2104253550987954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTGGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA363430.20552792819897125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC343110.19403650618922222No Hit
CCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA318110.17989843777171602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC292990.16569250662580576Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC274170.15504936872110708Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA264990.1498578699981988Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTACTTGCGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT247130.13975763392073237No Hit
TCCTGGGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA242950.13739374888132533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA234840.13280735948668632Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG229330.12969132920746795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT229010.12951036193172388No Hit
CGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT227170.1284698000961954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG223940.1266431616566536No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG222710.12594756869051232No Hit
ATCCGATTGGAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT198320.11215446914239326No Hit
TCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA192140.10865953862958572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTACTTGCGTGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG191000.10801484270974743No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA180780.10223520034067089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)