FastQCFastQC Report
Sun 2 Jul 2023
SRR3590770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5852156
Total Bases292.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG748121.2783664686997407TruSeq Adapter, Index 5 (100% over 49bp)
AACATAAATATATAAACGCAAACAATGGCCAGATCGGAAGAGCACACGTC191800.3277424593602768No Hit
ACTAAATCTCACACAGCCCATAGATCGGAAGAGCACACGTCTGAACTCCA103140.1762427385736129Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGAGAATGCAAGAAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT92860.1586765629624364Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT79520.1358815451946257TruSeq Adapter, Index 5 (100% over 48bp)
AACATAAATATATAAACGCAAACAACGGCCAGATCGGAAGAGCACACGTC62870.10743049228352763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)