FastQCFastQC Report
Sun 2 Jul 2023
SRR3590771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8888042
Total Bases444.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2000102.2503268998953874TruSeq Adapter, Index 5 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT311280.35022336753134153TruSeq Adapter, Index 5 (100% over 48bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT185140.20830234600601574TruSeq Adapter, Index 5 (100% over 43bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA158650.17849825642138056TruSeq Adapter, Index 5 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT157010.17665308062225626TruSeq Adapter, Index 5 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA156420.17598926737744938TruSeq Adapter, Index 5 (100% over 47bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA144110.16213919781207156TruSeq Adapter, Index 5 (100% over 47bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA130680.1470290081887552TruSeq Adapter, Index 5 (100% over 47bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT128670.14476754272763337TruSeq Adapter, Index 5 (100% over 43bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC124250.13979456892755457TruSeq Adapter, Index 5 (100% over 44bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC112200.1262370272327696TruSeq Adapter, Index 5 (100% over 44bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT111840.12583198864271794TruSeq Adapter, Index 5 (100% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT108010.12152282808744602TruSeq Adapter, Index 5 (100% over 48bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG106510.1198351672955641TruSeq Adapter, Index 5 (100% over 45bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG105740.11896883475573136TruSeq Adapter, Index 5 (100% over 45bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT103230.11614481569731556TruSeq Adapter, Index 5 (100% over 48bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT102710.11555975995612981TruSeq Adapter, Index 5 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT98660.11100307581804855TruSeq Adapter, Index 5 (100% over 43bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT94330.10613136166548268TruSeq Adapter, Index 5 (100% over 43bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT93960.10571507200348514TruSeq Adapter, Index 5 (100% over 43bp)
ACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT92280.10382489191657736TruSeq Adapter, Index 5 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)