FastQCFastQC Report
Sun 2 Jul 2023
SRR3590773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12562926
Total Bases628.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG3471882.763591857501986TruSeq Adapter, Index 5 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT522490.4158983345121988TruSeq Adapter, Index 5 (100% over 48bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT280270.2230929323312101TruSeq Adapter, Index 5 (100% over 43bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA268120.21342161849874786TruSeq Adapter, Index 5 (100% over 47bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT263750.2099431294906935TruSeq Adapter, Index 5 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA256110.20386174367340854TruSeq Adapter, Index 5 (100% over 47bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA254190.20233343729000713TruSeq Adapter, Index 5 (100% over 47bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA245360.19530481991217652TruSeq Adapter, Index 5 (100% over 47bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT232810.1853151089165056TruSeq Adapter, Index 5 (100% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT218420.17386077097007496TruSeq Adapter, Index 5 (100% over 48bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT210600.16763610642934615TruSeq Adapter, Index 5 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTAT208980.16634659791835119TruSeq Adapter, Index 5 (100% over 48bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC207550.16520832805988034TruSeq Adapter, Index 5 (100% over 44bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT207200.16492973054207277TruSeq Adapter, Index 5 (100% over 43bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC203590.16205619614411484TruSeq Adapter, Index 5 (100% over 44bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT188910.15037102025435795TruSeq Adapter, Index 5 (100% over 43bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT188470.15002078337482846TruSeq Adapter, Index 5 (100% over 43bp)
ACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT179590.14295236635159675TruSeq Adapter, Index 5 (100% over 43bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC176400.1404131489750079TruSeq Adapter, Index 5 (100% over 44bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG167840.1335994496823431TruSeq Adapter, Index 5 (100% over 45bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG165130.1314423089016046TruSeq Adapter, Index 5 (100% over 45bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC161730.1287359330143312TruSeq Adapter, Index 5 (100% over 44bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG160310.12760562308494056TruSeq Adapter, Index 5 (100% over 45bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT158600.12624447521222365TruSeq Adapter, Index 5 (100% over 43bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGT154130.12268638691336717TruSeq Adapter, Index 5 (100% over 46bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC152050.12103072166468225TruSeq Adapter, Index 5 (100% over 44bp)
AACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT150950.12015512946585852TruSeq Adapter, Index 5 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT150610.11988449187713118TruSeq Adapter, Index 5 (100% over 43bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA148350.11808554790500238TruSeq Adapter, Index 5 (100% over 47bp)
TGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC146250.11641396279815706TruSeq Adapter, Index 5 (100% over 44bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT144110.11471053797499087TruSeq Adapter, Index 5 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC142180.1131742716625092TruSeq Adapter, Index 5 (100% over 44bp)
AAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT139640.11115244967613437TruSeq Adapter, Index 5 (100% over 43bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT136730.10883611031379155TruSeq Adapter, Index 5 (100% over 43bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA136600.10873263123574874TruSeq Adapter, Index 5 (100% over 47bp)
CCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT135580.10792071846956672TruSeq Adapter, Index 5 (100% over 43bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT131720.10484818584460341TruSeq Adapter, Index 5 (100% over 43bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC131660.1048004262701221TruSeq Adapter, Index 5 (100% over 44bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT131180.10441834967427174TruSeq Adapter, Index 5 (100% over 43bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTA130160.10360643690808972TruSeq Adapter, Index 5 (100% over 47bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG126000.10029510641071991TruSeq Adapter, Index 5 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)