FastQCFastQC Report
Sun 2 Jul 2023
SRR3590775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347995
Total Bases317.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1442642.2725915820664637TruSeq Adapter, Index 6 (100% over 49bp)
AACATAAATATATAAACGCAAACAATGGCCAGATCGGAAGAGCACACGTC215880.34007588222738044No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC164230.25871160894109085TruSeq Adapter, Index 6 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT164170.25861709090823165TruSeq Adapter, Index 6 (100% over 48bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA84950.13382178152314234TruSeq Adapter, Index 6 (100% over 47bp)
CTGAGAATGCAAGAAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT77030.12134540118572872Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACATAAATATATAAACGCAAACAACGGCCAGATCGGAAGAGCACACGTC72260.1138312175734228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)