FastQCFastQC Report
Sun 2 Jul 2023
SRR3590776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5429976
Total Bases271.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG249870.4601677797470928TruSeq Adapter, Index 6 (100% over 49bp)
AGATCGGAAGNGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG89670.1651388514424373TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)