FastQCFastQC Report
Sun 2 Jul 2023
SRR3590777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8715486
Total Bases435.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1570341.8017813349708782TruSeq Adapter, Index 6 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT249810.28662773366855276TruSeq Adapter, Index 6 (100% over 48bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA124730.14311307481877658TruSeq Adapter, Index 6 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT117030.13427822613678686TruSeq Adapter, Index 6 (100% over 43bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA116800.13401432805927288TruSeq Adapter, Index 6 (100% over 47bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA116650.13384222061741594TruSeq Adapter, Index 6 (100% over 47bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT114400.13126060898956182TruSeq Adapter, Index 6 (100% over 43bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA104110.11945403847817551TruSeq Adapter, Index 6 (100% over 47bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT91590.10508880399784934TruSeq Adapter, Index 6 (100% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT90640.10399879019942204TruSeq Adapter, Index 6 (100% over 48bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC87990.10095822539328272TruSeq Adapter, Index 6 (100% over 44bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT87200.10005179286616948TruSeq Adapter, Index 6 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)