FastQCFastQC Report
Sun 2 Jul 2023
SRR3590781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6228853
Total Bases311.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG3269735.24932921036987TruSeq Adapter, Index 7 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT391590.6286711213123829TruSeq Adapter, Index 7 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC247550.39742469440200306TruSeq Adapter, Index 7 (100% over 50bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA153960.24717231246266366TruSeq Adapter, Index 7 (100% over 47bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT147910.2374594487941841TruSeq Adapter, Index 7 (100% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT142860.22935201713702347TruSeq Adapter, Index 7 (100% over 48bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA142820.22928779985657072TruSeq Adapter, Index 7 (100% over 47bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA114010.1830353036104721TruSeq Adapter, Index 7 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT110940.17810662733572297TruSeq Adapter, Index 7 (100% over 43bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG109740.17618010892214023TruSeq Adapter, Index 7 (100% over 45bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG96090.15426596196763673TruSeq Adapter, Index 7 (100% over 45bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT89670.14395908845496916TruSeq Adapter, Index 7 (100% over 48bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT88870.142674742845914TruSeq Adapter, Index 7 (100% over 43bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG88870.142674742845914TruSeq Adapter, Index 7 (100% over 45bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT88440.14198440708104687TruSeq Adapter, Index 7 (100% over 43bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT87940.1411816910753874TruSeq Adapter, Index 7 (100% over 46bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC87900.14111747379493464TruSeq Adapter, Index 7 (100% over 44bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC85850.13782633817173082TruSeq Adapter, Index 7 (100% over 44bp)
CTGAGAATGCAAGAAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT85810.13776212089127807Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT85280.13691124192527904TruSeq Adapter, Index 7 (100% over 43bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA85060.13655804688278886TruSeq Adapter, Index 7 (100% over 47bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT79900.12827401770438313TruSeq Adapter, Index 7 (100% over 43bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT79880.12824190906415675TruSeq Adapter, Index 7 (100% over 43bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT79370.1274231387383841TruSeq Adapter, Index 7 (100% over 43bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT79070.12694150913498842TruSeq Adapter, Index 7 (100% over 43bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG78330.1257534894466124TruSeq Adapter, Index 7 (100% over 45bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT76090.12215732174125798TruSeq Adapter, Index 7 (100% over 43bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT75990.1219967785401261TruSeq Adapter, Index 7 (100% over 43bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA75830.12173990941831506TruSeq Adapter, Index 7 (100% over 47bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC74760.12002209716620381TruSeq Adapter, Index 7 (100% over 44bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC74180.11909094659963881TruSeq Adapter, Index 7 (100% over 44bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT74070.11891434907839372TruSeq Adapter, Index 7 (100% over 43bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA72840.11693966770447144TruSeq Adapter, Index 7 (100% over 47bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG71430.11467600856851173TruSeq Adapter, Index 7 (100% over 45bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC71050.11406594440421054TruSeq Adapter, Index 7 (100% over 44bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT70300.11286187039572133TruSeq Adapter, Index 7 (100% over 43bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT69690.11188255686881679TruSeq Adapter, Index 7 (100% over 46bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA68780.11042161373851654TruSeq Adapter, Index 7 (100% over 47bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC65520.1051879053816168TruSeq Adapter, Index 7 (100% over 44bp)
ACTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT63970.10269948576407245TruSeq Adapter, Index 7 (100% over 43bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGT63600.10210547591988443TruSeq Adapter, Index 7 (100% over 46bp)
TGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC62830.10086929327116888TruSeq Adapter, Index 7 (100% over 44bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT62570.10045188094822595TruSeq Adapter, Index 7 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)