FastQCFastQC Report
Sun 2 Jul 2023
SRR3590782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978934
Total Bases198.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG466871.1733544713232238TruSeq Adapter, Index 8 (100% over 49bp)
AACATAAATATATAAACGCAAACAATGGCCAGATCGGAAGAGCACACGTC130350.32760030701690457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC52700.13244753494277614TruSeq Adapter, Index 8 (100% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT49700.12490782707127086TruSeq Adapter, Index 8 (100% over 48bp)
CTGAGAATGCAAGAAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT49110.12342501785654147Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)