FastQCFastQC Report
Sun 2 Jul 2023
SRR3590784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7085293
Total Bases354.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1034701.460348922761557TruSeq Adapter, Index 8 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT123810.17474224425157858TruSeq Adapter, Index 8 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC79190.11176672580795177TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)