FastQCFastQC Report
Sun 2 Jul 2023
SRR3590785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3590785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12964050
Total Bases648.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG6127234.726323949691647TruSeq Adapter, Index 8 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT712030.5492342285011242TruSeq Adapter, Index 8 (100% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC485150.37422718980565484TruSeq Adapter, Index 8 (100% over 50bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA318380.2455868343611757TruSeq Adapter, Index 8 (100% over 47bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA274140.2114616959977785TruSeq Adapter, Index 8 (100% over 47bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT261080.2013876836328154TruSeq Adapter, Index 8 (100% over 48bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT241280.18611467866908876TruSeq Adapter, Index 8 (100% over 48bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA233740.18029859496067974TruSeq Adapter, Index 8 (100% over 47bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT229950.17737512582873408TruSeq Adapter, Index 8 (100% over 43bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC213630.16478646719196546TruSeq Adapter, Index 8 (100% over 44bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA208430.1607753749792696TruSeq Adapter, Index 8 (100% over 47bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG206110.15898581076129759TruSeq Adapter, Index 8 (100% over 45bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT202510.15620890076789276TruSeq Adapter, Index 8 (100% over 46bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG200960.15501328674295456TruSeq Adapter, Index 8 (100% over 45bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT199890.15418792738380369TruSeq Adapter, Index 8 (100% over 48bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC197350.15222866311067915TruSeq Adapter, Index 8 (100% over 44bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT194680.15016912153223722TruSeq Adapter, Index 8 (100% over 43bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA194370.1499299987272496TruSeq Adapter, Index 8 (100% over 47bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT184070.14198495069056352TruSeq Adapter, Index 8 (100% over 43bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT183800.14177668244105818TruSeq Adapter, Index 8 (100% over 43bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC183140.1412675822756006TruSeq Adapter, Index 8 (100% over 44bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT177300.13676281717518832TruSeq Adapter, Index 8 (100% over 43bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT172420.13299856140635063TruSeq Adapter, Index 8 (100% over 43bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT170080.1311935699106375TruSeq Adapter, Index 8 (100% over 43bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT169220.13053019696776857TruSeq Adapter, Index 8 (100% over 43bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT168910.13029107416278093TruSeq Adapter, Index 8 (100% over 43bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC168810.13021393777407522TruSeq Adapter, Index 8 (100% over 44bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG168680.13011366046875783TruSeq Adapter, Index 8 (100% over 45bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG166250.12823924622320956TruSeq Adapter, Index 8 (100% over 45bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG160200.12357249470651532TruSeq Adapter, Index 8 (100% over 45bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC159420.12297083087461093TruSeq Adapter, Index 8 (100% over 44bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC156460.12068759376892252TruSeq Adapter, Index 8 (100% over 44bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT155850.12021706179781783TruSeq Adapter, Index 8 (100% over 43bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT155760.1201476390479827TruSeq Adapter, Index 8 (100% over 43bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGT148530.11457067814456132TruSeq Adapter, Index 8 (100% over 46bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA146100.11269626389901304TruSeq Adapter, Index 8 (100% over 47bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT145560.11227972740000232TruSeq Adapter, Index 8 (100% over 43bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG145400.1121563091780732TruSeq Adapter, Index 8 (100% over 45bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT142500.1099193539056082TruSeq Adapter, Index 8 (100% over 43bp)
GGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG141850.10941796737902122TruSeq Adapter, Index 8 (100% over 45bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTA133140.10269938792275561TruSeq Adapter, Index 8 (100% over 47bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTC130010.10028501895626753TruSeq Adapter, Index 8 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)