FastQCFastQC Report
Sun 2 Jul 2023
SRR3591805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3591805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36965332
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC29408417.955673169660697No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT13905573.761786854775172No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG10273112.7791201767104377No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG6426821.7386074065289068No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC6252081.6913360875536028No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6068241.6416030025105686No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG5455991.4759748404261592No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT5376541.4544817289886642No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4396751.1894252701423054No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT4390311.1876830972328343No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG3445240.9320192227679708No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA3093300.836811096407845No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGA2505830.6778865126924871No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG2278770.6164613914464504No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1929930.5220918886918153No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG1672130.4523508675642356No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1537870.41603034973417796No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAATA1481370.40074575821475106No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1394420.37722371869945603No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCG1362030.36846145464079694No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1312180.3549758460170194No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1269860.34352728118335313No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG1153190.3119652760050958No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1012010.273772733868588No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC964130.2608200570199126No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCA945670.2558261887110874No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG863900.233705462188193No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT816020.22075278533951756No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATAGATC814850.22043627255938078No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG786200.21268576730218464No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGCTGTAGGCACCATCAAT735700.19902431824499778No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGAT724870.19609454610065455No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG701410.18974805907329603No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT694470.18787062429197174No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA689700.18658022603449093No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTGTAGGCACCATCAATAGATCG677210.1832013844755946No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCCTGTAGGCACCATCA670880.18148896917793136No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG666610.1803338327923039No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT664270.17970080723203027No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGATCG621950.16825224239836395No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC616650.16681846655671861No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGATCGG616550.16679141418234794No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT610690.16520614504422684No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA605640.16384000013850816No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCG585050.15826991625558778No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT575650.1557269930647451No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATAG571240.15453398335499868No Hit
GTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC494120.13367119224034021No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA484030.13094160766633992No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT483140.13070084153444098No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT478360.12940773803952307No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT475790.12871249201819696No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA465160.12583682462259504No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG453990.12281507440539152No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG419650.11352528904650444No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATAGAT418220.1131384400930039No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG414060.11201306131918415No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA412550.11160457046618708No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATAG409390.110749715436074No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAATA398360.10776583854298942No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGAT392010.10604801277045205No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG388960.1052229153521467No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTGTAGGCACCATCAATAGATC374560.10132737344277065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)