FastQCFastQC Report
Sun 2 Jul 2023
SRR3591806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3591806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35758954
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA33671089.416125538795123No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT21527256.02010058795344No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA16261064.5474093006188046No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA11769643.291382628250256No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC9449202.64247102977341No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA8928122.496750883708735No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT5167631.445128959868345No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG5068431.4173876562496766No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3817191.067478092340173No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA3649911.020698200512241No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT3299420.9226835885635806No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT3047070.8521138509812115No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT2661780.7443674107469698No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG2025930.5665518068565428No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1899260.5311285111975032No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1787890.49998386418126217No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1678850.46949080221977413No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA1648120.4608971504032249No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT1618170.45252162577238697No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1579770.4417830566296766No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1499480.41932993901331683No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC1429360.3997208643183467No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC1423600.39811007894694006No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1394270.3899079374637189No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1349830.37748028088293634No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1262100.3529465654951764No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAGCTGTAGGCACCATC1206480.3373924192525318No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1169250.3269810408883884No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1109850.31036981674575825No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1054610.2949219375935884No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG988000.27629443523431924No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA984230.2752401538367146No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA970050.2712747134605783No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG952680.2664171888249304No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA922060.2578542985345712No Hit
TATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA900810.25191173097512864No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA882040.24666269600615276No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA824780.23064992337303827No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG660030.18457754664747744No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA659170.18433704744271884No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA637740.17834414284041977No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA623530.17437031295714076No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG607830.16997980421910552No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA593070.1658521667048762No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG584900.16356742425966933No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA542180.15162076608840402No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA541810.1515172955003102No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA535920.14987015559795178No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA528810.1478818424051218No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA523280.14633537658847626No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA515030.1440282621242221No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT492960.13785638136954453No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC490410.13714327326241144No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA489810.13697548311955657No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA445770.1246596866340106No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA399060.11159722401276055No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAA381880.10679283292235No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA378760.10592032417950481No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG372860.10427038777476545No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG368580.10307348475573419No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)