FastQCFastQC Report
Sun 2 Jul 2023
SRR3591807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3591807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34179565
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA30835009.021472332956842No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA21523686.2972363750094535No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA11188473.273438383431738No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC10044882.938855424286412No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG8102172.37047194720003No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT7384942.1606301894128843No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA6713981.9643257601435242No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4942711.4461009085399419No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT4691461.3725920736557062No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT4266141.2481551476737636No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA3843551.1245169445544436No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG3440621.006630716335916No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA3144450.919979525778049No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG3087880.9034287007456063No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG2101080.6147181802928153No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT1882190.5506769907691921No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1704350.49864590143262505No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1514360.4430600564986711No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC1512560.4425334260397989No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1497730.4381945762036469No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1413830.4136477453706623No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG1376020.4025855800095759No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1332210.3897679797855824No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1301700.3808415935076997No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1242010.3633779423465454No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1177490.3445011661207508No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA1102560.3225787104078124No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1083040.3168676956538212No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1049100.30693778577930997No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1006650.2945180841242421No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG986800.28871052045279105No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA964030.2820486451480585No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG943890.2761562354582336No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA937000.2741404110906619No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA925750.2708489707227111No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA864400.25289964924948577No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA859000.25131975787286936No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA776580.22720593430606856No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG699310.20459885899659636No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA676300.19786676629734756No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA673270.19698027169157947No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT597880.1749232326391515No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA527750.15440512481653876No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG509570.1490861571819302No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT502100.14690064077761084No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA488820.14301527828104307No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA465010.13604912760007332No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG437490.12799753302887265No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA417790.12223385522899428No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA415990.12170722477012215No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA412190.12059544935694765No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA406520.11893656341150041No Hit
TATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA402530.11776919922766717No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA385070.11266088377660745No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC378340.11069187100537997No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA373990.1094191807297723No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG360880.10558355555432025No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA356470.10429331093008351No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAA355710.10407095584744862No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT354550.10373157177395323No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA351840.10293870036087352No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA345150.10098139048873209No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG342620.10024118212153957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)