FastQCFastQC Report
Sun 2 Jul 2023
SRR3591812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3591812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22280204
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG7612123.416539633120056No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA3598271.6150076543284793No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA3235881.4523565403620182No Hit
GTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGC3166701.4213065553618809No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2596631.1654426503455713No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2004640.8997404153031993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1904400.8547498039066429Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1835960.82403195231067No Hit
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGA1780170.7989917866102123No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1588240.7128480511219736No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG1440160.6463854639751054No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1406910.6314619022339293No Hit
CACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAG1396130.6266235264273163No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1366420.6132888190790353No Hit
CAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1293770.5806813977107211No Hit
GACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1263390.5670459749829939Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG1103130.4951166515351475No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1065660.4782990317323845No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG890060.3994846725819925No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC861190.38652698153033066No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG850590.3817693949301362No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG811280.36412592990620735No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT798780.35851556834937415No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC766920.3442158788133179No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA617340.2770800482796298No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC572220.25682888720408487No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG571470.2564922655106749No Hit
CCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT560370.251510264448207No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA519300.23307686051707607No Hit
GTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAG515890.231546353884372No Hit
TGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAAG509490.22867384876727342No Hit
TCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT491700.2206891821995885No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA484710.21755186801700738No Hit
TTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATAGATCG477620.21436967094197162No Hit
TGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGAG477090.2141317916119619No Hit
ACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC473710.21261474984699422No Hit
TAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC473300.21243072998793008No Hit
TTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA450090.20201341064920234No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA450060.2019999457814659No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC435990.19568492281309452No Hit
ACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGC420780.18885823487073994No Hit
TTGTGGCGTCGCTGAACCATAGCTGTAGGCACCATCAATAGATCGGAAGA417990.1876060021712548No Hit
GGTTAAGGCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC378660.169953560568835No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA378360.169818911891471No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA362850.16285757527175246No Hit
ACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG360820.16194645255492277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGC343750.15428494281291139No Hit
GTCTAGTCGGTTATGGCACTGTAGGCACCATCAATAGATCGGAAGAGCAC341170.15312696418758107No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA339940.15257490461038867No Hit
CGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAGAG335290.15048785011124674No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA334960.15033973656614635No Hit
AGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT333100.14950491476648958No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA332780.14936128951063465No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC331890.14896183176778813No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC327450.14696903134280098No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA325900.14627334650975368No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA322880.1449178831576228No Hit
AGCGGAGAATTTGGACAAACTTGGTCACTGTAGGCACCATCAATAGATCG317750.14261539077469848No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA310500.13936138107173526No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAG309070.13871955570963354No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT303850.13637666872350002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC302440.13574381993988924No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA299790.13455442328984063No Hit
GTTTAGTGGTAAAATCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC291490.1308291432161034No Hit
GAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAGATCGGAAGAGC286930.12878248332017067No Hit
TGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAGA286320.12850869767619721No Hit
TTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT276210.12397103724903058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAG275010.1234324425395746No Hit
CCTGCGGAAGGATCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG270820.12155184934572413No Hit
AATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGC270540.12142617724685108No Hit
GTCTAGTCGGTTATGGCATCTGCTGTAGGCACCATCAATAGATCGGAAGA269060.12076191043852201No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA264710.11880950461674408No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG264670.11879155145976222No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG261110.11719372048837613No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA259400.11642622302740137No Hit
TTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC258940.1162197617221099No Hit
GGTTAAGGCACCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT257330.11549714715358979No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA254640.1142897973465593No Hit
AATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG254610.1142763324788229Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT250150.11227455547534483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT249290.11188856260023472No Hit
ATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG248660.11160580037777032No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG241860.10855376369085311No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA241510.10839667356726176No Hit
ATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGATCGGAAGA235860.10586079014357319No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT234210.10512022241807122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC231070.10371089959499474No Hit
TTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG230830.10360318065310353No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG227330.10203227941719027No Hit
CCTGCGGAAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC226130.10149368470773426No Hit
TTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATAGATCGGAAGA223200.10017861595881258No Hit
TATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC223120.10014270964484885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)