FastQCFastQC Report
Sun 2 Jul 2023
SRR3591814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3591814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14832781
Total Bases756.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA5723763.8588582950156143No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3729342.5142554184545705No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3378052.2774218806304765No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT3340332.251991720231021No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2550791.7196977424530167No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA2492601.6804670681782465No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1870331.260943581652018No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1854581.2503252087386716No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1812641.222049998580846No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG1285250.8664929388494308No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1265570.8532250290758018No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1028200.6931943510795447No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1018090.6863783669427871No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT831260.5604208678062461No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA713260.4808673437570473No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG710470.4789863748409688No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA709000.47799532670238976No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG688570.46422178012336324No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT680850.45901709193980555No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA656750.44276929592636743No Hit
AACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATC637070.42950138615273836No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG605330.40810283654831825No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG598670.4036127817163888No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG597080.4025408316889463No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA593850.4003632225137012No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT577970.3896572058874192No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT567000.38226142488047254No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA563480.3798883028071405No Hit
ATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGGAA472620.31863208928925735No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG469490.316521898354732No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA436250.294112075139517No Hit
CTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGG415770.2803048194401306No Hit
TCTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG400700.27014489056367785No Hit
TATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCGGA386010.26024115100195977No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA379860.25609492919770066No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA370950.250087963949579No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT370460.24975761457005263No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA364440.24569903647872912No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT348280.23480424877843206No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA338160.22798152281760248No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA329520.22215658681942382No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA326300.21998571946825074No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA308060.20768863236098478No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG291710.19666575000332034No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG270530.18238656661889635No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG270130.18211689365601771No Hit
ATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATCGGA267510.1803505357491626No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA257780.1737907409271397No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT253070.1706153417892437No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA251030.16924000967856262No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG243860.164406121818963No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG242320.16336788091188026No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA242080.16320607713415305No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA229700.15485969893305915No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG229610.15479902251641145No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG219200.14778078365749484No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA217690.146762768222628No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT214120.14435593702893612No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA212610.14333792159406925No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA207410.13983217307664692No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG207130.13964340200263187No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG205910.13882089946585202No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC205570.1385916774474052No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA201780.13603652612413006No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC199260.13433758645799462No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA198880.13408139714325992No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA195850.13203862444945422No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT190820.1286474869412553No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT190740.1285935523486796No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAA185440.12502038559053763No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA184490.12437991230370084No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA180900.12195959746186505No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA176590.1190538712868477No Hit
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG176000.1186561036666017No Hit
AAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGATCG174090.11736841526885618No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA171930.11591218126931155No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATA171000.1152851916306187No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA170860.11519080609361117No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC169000.1139368268162255No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA164180.11068726761353789No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATAGATC163580.11028275816921992No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA163100.10995915061376554No Hit
AAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA160370.10811863264211882No Hit
TTTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAA157470.10616350366124869No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG157100.10591405617058594No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC156130.10526009923560525No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC155770.10501739356901446No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA154030.10384431618049239No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATAGATC153360.10339261396767065No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG152900.10308249006036022No Hit
TTGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC151480.10212515104214105No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA151390.10206447462549335No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGA151390.10206447462549335No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT151200.10193637996812602No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG149410.10072959345924408No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG148470.10009586199647927No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT148380.1000351855798316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)