FastQCFastQC Report
Tue 4 Jul 2023
SRR3591815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3591815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23385198
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA9212283.9393636949321533No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA6041132.5833135986276448No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4765882.037990014025111No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3737691.5983144551523574No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA3649681.560679537543364No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT3449331.4750056852201978No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2926671.251505332561221No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG2133270.9122308906685331No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2070810.885521687693215No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2064720.882917476259983No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1932800.8265057238343674No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1842460.7878744494701306No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT1779370.7608958453120646No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1685530.720767897710338No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1543210.6599088876647528No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1158680.49547581337562335No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1110380.4748217226982641No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA1059950.453256799450661No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1013770.43350926513429566No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA977430.41796952071990157No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT958850.41002432393345567No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG951750.4069882153659764No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG935940.40022752854177246No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA922390.394433265008062No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG777970.3326762510199828No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT744750.3184706838915796No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA735630.3145707810556062No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG729970.3121504466201227No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG714260.30543252188841846No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG648720.27740624646410944No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA625120.2673143926341783No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT624650.26711341079942963No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG599000.2561449340732544No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA590080.25233055542228033No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG567060.24248672172884744No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG561850.24025881670961263No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA548020.23434481931690296No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA534120.22840088845944342No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT524670.22435987071822097No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA522250.22332502807972804No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA477300.20410346750110903No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC462650.19783882094990174No Hit
AAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGATCG456730.19530730507391897No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATAGATC454210.19422970034292633No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC450800.19277151298868625No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA438280.18741769900772273No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG433350.1853095278474871No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG432710.18503585045548898No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA413350.1767571093475454No Hit
AGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAA408200.1745548615838104No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG386760.1653866689518729No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA376310.16091803028565335No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAG376240.16088809682090355No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA357760.1529856621269574No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT355250.15191233360521472No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA352930.15092025305922147No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA350450.1498597531652287No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG349880.14961600923798038No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA346900.14834169888148904No Hit
GATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCGGA345050.14755060017024446No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC344530.14732823728924596No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG335560.1434924775920221No Hit
TTGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC333670.1426842740437776No Hit
AAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAGATC330300.14124319152653744No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT327630.1401014436567952No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC325900.13936165945655027No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA319180.13648804684056984No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG317700.13585516787157415No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA316440.1353163655060778No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA314350.13442263777283392No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA305380.13058687807561006No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA301530.12894053751437126No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG296550.1268109853078858No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGA289040.12359955216115767No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATAGATC288130.12321041711941033No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAG287560.12296667319216198No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA285720.12217985069016735No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA284240.12154697172117165No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT281870.12053351012892856No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG280770.12006312711143176No Hit
ACTATGCCGACTAGGGATCGGGTGGTGCTGTAGGCACCATCAATAGATCG278590.11913091349493812No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA275460.11779245999969723No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG270420.11563725053771194No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC269820.11538067798271368No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT264470.11309290603397927No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT260880.11155774691323975No Hit
TATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGG259890.11113440219749263No Hit
TAGCGGAGAATTTGGACAAACTTGGTCACTGTAGGCACCATCAATAGATC259460.11095052519974388No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG258160.11039461799724766No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGA257640.11017225511624917No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA256610.10973180556350219No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA255570.10928707980150522No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA252810.10810684604851326No Hit
AGGTCCAGACACAATAAGGATTGACAGACTGTAGGCACCATCAATAGATC251910.10772198721601586No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGAT251170.10740554773151804No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA246620.10545987252278129No Hit
TTCTTAGTTGGTGGAGTGATTTGTCTGCTTACTGTAGGCACCATCAATAG246260.10530592898978235No Hit
CGCGCGCTACACTGACGGAGCCAGCGCTGTAGGCACCATCAATAGATCGG245270.10488258427403523No Hit
GATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATCAATAGATCG242260.103595445289794No Hit
GGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGATC242070.10351419731404454No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT241100.10309940501679739No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA238230.10187213296205574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)