FastQCFastQC Report
Mon 10 Jul 2023
SRR3623932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168869433
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2289253213.556350366854137No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT81061764.800262460761623No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA76885124.552932915929196Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA55309873.2753038260038454No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT38570502.2840427254824736No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT35444712.0989417309170455No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT32224081.9082245630563586No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA22008951.303311653802971No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT18830021.1150638493586935No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA16023680.9488798366487083No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA12598480.7460485758840678No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT9994710.5918602213818056No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT9984360.591247321828812No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC9194990.5445029237470111No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA8648780.5121578160329348No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA8172870.48397568789136636No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7890330.46724441835486No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC7629160.4517786235475783No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7152900.4235757693341695No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA6022470.356634702504153No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT5828590.34515364305155216No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT5316320.31481837213251024No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT4998690.2960091658506368No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA4391370.2600452859932324No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC4222230.2500292637330049No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG4068750.2409405851442635No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT3988620.23619549903978182No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3776290.22362187951445303Illumina PCR Primer Index 6 (96% over 29bp)
CCTCCAGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3760470.22268506106726846No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC3757520.22251036989032824No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATCT3725880.22063673299595907No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT3489780.2066555171059288No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3201440.18958078695035355No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA3106620.1839657980020576No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3100720.18361641564817713No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATC3042870.1801906920597051No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2989770.17704625087478087No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2857740.1692277844031134No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCT2788480.16512639087264538No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2752110.1629726559216907No Hit
GTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2722820.16123817979539257No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2647570.15678207434971372No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA2610250.15457208291804947No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA2458920.14561072162775604No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA2399530.14209380332318639No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA2377730.1408028651342721No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAT2279010.13495692852832636No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAT2191120.1297523158024697No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATA2129790.12612051584255632No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAT2125460.1258641047252169No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2113830.12517540696663557No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2092930.12393776439102511No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1965370.11638399946543317No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCT1951940.11558871048024423No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT1946590.1152718976678272No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1942080.11500482742782703No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1869240.11069143579110613No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA1826480.10815930198569447No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1817820.1076464797510157No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCA1788250.10589542276724527No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1742210.10316905606001531No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCGTATGCCG1708070.10114737579535783No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1702820.10083648471775233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)