FastQCFastQC Report
Thu 6 Jul 2023
SRR3623933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90160106
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1245896513.818711570725084Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7743290.8588377214197153Illumina PCR Primer Index 6 (96% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6333800.7025058289084088No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT5294850.5872719359935091No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA4493640.49840668998326154No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT4357850.48334570502834145No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT4325160.4797199328936015No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA4260300.47252606380032425No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3919880.4347687878716558No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3646450.4044416274310947No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA3606600.4000217124855643No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA3583630.3974740224906124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG3565960.39551417563772606No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG3408360.3780341606963062No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA3103850.3442597993396326No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC3021120.335083900633391No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2937230.3257793419186974No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC2455900.27239320237711345No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC2415510.26791339397937264No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT2397390.26590363591631094No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAA2099470.23286019650420553No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT2059960.2284779922508077No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1923970.21339482453580969No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAA1842270.20433316704396953No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCTGTAGGCACCATCAATATCTC1736190.19256743109862803No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAA1700380.18859560790667215No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA1433120.15895278561451556No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATAT1397050.15495212483445836No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1395890.1548234648260063Illumina PCR Primer Index 6 (96% over 29bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC1376700.15269502899652757No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1371280.1520938761984153No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1349620.14969148328197396Illumina PCR Primer Index 6 (100% over 28bp)
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATA1292870.14339712510985733No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT1259200.13966265745073547No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC1244440.1380255697569832No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA1232610.13671345949837282No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAA1227590.1361566722204164No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1221070.13543351424187544No Hit
CTGTAGGCAACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1198880.13297233701122757Illumina PCR Primer Index 6 (100% over 28bp)
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAAT1198760.1329590273551808No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT1125370.12481906354457925No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1089670.12085944087066623No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA1080600.11985345270113149No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAA1057580.11730021701616011No Hit
TTGCCAAGAATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1055070.11702182337718192No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT1042290.1156043450082013No Hit
AAATGGATTTTTGGAGCAGGGAGATGGACTGTAGGCACCATCAATATCTC1011900.11223367461435771No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT989220.10971814962151885No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG981710.10888518698059205No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA958960.10636189802172592No Hit
TATAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCA937320.10396172338129239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)