FastQCFastQC Report
Fri 8 Sept 2023
SRR3623934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265685072
Total Bases13.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA230013748.65738290331946Illumina PCR Primer Index 6 (96% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA139920765.266414064844411No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT105394023.96687774765155No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA80504003.0300535665775006No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA32096521.2080663681397952No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT29981321.1284533140800623No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT25045540.9426777278627081No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT24193760.9106179665224097No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC16569520.623652652942428No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA16220100.6105009919413161No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA15857760.5968630409163522No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT14794860.5568570295887757No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14331560.5394190908851665Illumina PCR Primer Index 6 (96% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT13769400.5182602054510612No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11298080.42524331212707356No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA10888320.4098205412158045No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT10586620.39846499166501914No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA10405380.3916433814542655No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT9134900.3438243605948625No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT7944800.29903072612224146No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG7709460.29017287053297447No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT7345880.27648824771005576No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC7143860.2688845084980913No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG6823660.2568326458326571No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA6802720.25604449466396817No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT6232640.2345875119396998No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5748860.21637873579890105No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA5745800.21626356184588344No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT5681680.21385017822905758No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT5528000.20806588636639697No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC5100100.19196035221730484No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT5039140.18966590640817035No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC4955600.18652158221369697No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA4639020.1746059710874535No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4457380.1677693054580048No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC4390300.16524451174283514No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT4249260.15993597111093996No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATC4132760.15555108041598964No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA4123280.15519426699291558No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA3815160.14359707797207366No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAA3537700.13315388679421175No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATC3385760.12743508600287487No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA3348040.12601536001992616No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATC3313360.12471005521906026No Hit
TTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT3267080.12296814327603624No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC3231320.12162218884469353No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT3166800.11919374980917258No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC3144920.11837021840655014No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT3140820.11821590036492528No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAA3135720.11802394377656265No Hit
CGGTGGCGGCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATATCT2977500.11206877291171256No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC2963800.1115531248214051No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT2946300.1108944502534941No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATC2932880.11038934095627322No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA2894500.10894477353247758No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2878120.10832825413691288No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATCT2853060.10738503215566436No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA2850180.10727663314105958No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA2807720.10567850044657383No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2795620.1052230740310468No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT2752420.10359708881197512No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG2691160.10129135143881927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)