FastQCFastQC Report
Fri 7 Jul 2023
SRR3623935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158817140
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2593800416.3319928818766Illumina PCR Primer Index 6 (96% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA146145749.202139013459126No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT88473595.570783480926555No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA53383533.3613204468988673No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT23080341.4532650569075858No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT20998281.3221671162193198No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT14065160.8856197763037416No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA13965900.8793698211666574No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13867370.8731658308416836Illumina PCR Primer Index 6 (96% over 29bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC10574450.665825489616549No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA9742350.6134319003603768No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT8997890.5665566071772858No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA8756710.5513705888419852No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT7722260.48623593146180566No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC7707880.48533048762872827No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7612040.47929587448810623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT7459470.4696892287570473No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT7348810.4627214669650896No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA7221410.4546996627693963No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA5527540.34804429798949915No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC5490560.3457158339458827No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5382070.3388847072803351No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT5324890.33528434021667936No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG5107730.3216107530962968No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA4825200.30382111149967816No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4711650.2966713794241604No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA4514580.2842627691192525No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT3785010.23832503217222023No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG3586770.22584275223694372No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3505960.22075451050182618No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3288220.20704440339373947No Hit
CCTCCAGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3166670.19939094734988932No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTCG3136990.19752213142737615No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCT2984210.18790226294214843No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2829810.1781803903533334No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2753830.17339627196409657No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT2749180.17310348240750337No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2664360.16776274903325925No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC2606110.16409500888883907No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2529250.1592554808630857Illumina PCR Primer Index 6 (100% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2337330.14717114286279176Illumina PCR Primer Index 6 (96% over 29bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCT2146520.13515669656310395No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2136740.13454089401181762No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC2053120.12927571923282336Illumina PCR Primer Index 6 (96% over 29bp)
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC2009480.1265279049855702No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA2005810.1262968216150977No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2002040.1260594416950211No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT1924440.12117331920219696No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA1918930.120826379319008No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1897930.11950410390213549No Hit
TCCTCACGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1868740.11766614107268272No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1855930.11685955306839048No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1853220.11668891657411788No Hit
TTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1839430.11582062238370493No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG1758150.1107027868654479No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT1757450.11065871101821881Illumina PCR Primer Index 6 (96% over 29bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATC1737330.10939184523786286No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1718540.10820872356724218No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1686500.10619130907407097No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT1676870.10558495134719087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)