FastQCFastQC Report
Sun 2 Jul 2023
SRR3623936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59607727
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA51735528.679331120946786No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT35287015.9198717642764676No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA26629664.467484559510212No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA9297551.5597893877080735No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT8306491.3935257085042012No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT7981081.3389337929292289No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7909571.3269370261342124No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5086880.8533927153437674Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT4826680.8097406566098385No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4580990.7685228460397425No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA4344130.728786387040056No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT3903780.6549117365270446No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT3833310.6430894437561762No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3716940.6235668070349335No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3333610.5592580304228008No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT2967610.49785659500151713No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2592550.4349352224083297No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA2583380.4333968312531025No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA2520070.42277572503309846No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2486590.41715900356341384No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT2289150.38403578113287223No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2142610.3594517200764928No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2007250.3367432547797033No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1970900.3306450521087643No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG1852120.310718105389256No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1631380.2736859937638622No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1617310.2713255615333227No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA1478070.24796617391567374No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1406040.2358821701085834No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1319370.22134210888464173No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1261700.21166718871867066No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA1188240.19934328312837696No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1165780.19557531526072114No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1140970.19141310320388497No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC1120930.18805112296934254No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAA1079510.18110235943068254No Hit
CCTGCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1041800.17477599842047326No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC957080.1605630759918089No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT942430.15810534094011No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCA887170.14883473077240472No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT884720.1484237102347486No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT865560.14520936186679287No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA865430.1451875526137744No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT818050.13723891870595903No Hit
TTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT786720.13198288872850328No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC782220.13122795304709403No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA782170.13121956487285616No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCTGTAGGCACCATCAATATCTC779530.13077666927309609No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT762580.12793307820645466No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA761020.1276713671702328No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC754400.12656077290113746No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA751650.12609942331805404No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT732280.12284984461829924No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT710250.11915401504908919No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG706360.1185014150933821No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATC696590.11686236584730031No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA693080.1162735160158011No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT692680.11620641062189807No Hit
CGTGGGGGTCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA679000.11391140615041402No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAT660120.11074403155819043No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT636640.10680494493608186No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC635750.1066556354346476No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG629470.10560208075036982No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT628310.10540747510805101No Hit
TGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA609930.10232398225820621No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA606310.10171667844338368No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT603710.10128049338301393No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA600670.1007704923893508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)