FastQCFastQC Report
Sun 2 Jul 2023
SRR3623937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38340949
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGG4291991.119427169108412No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4231531.1036581280239048No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3847621.0035275861325184Illumina PCR Primer Index 6 (96% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2977590.7766083202583223No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2657290.6930683953597497No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2350560.613067767310611No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC2278370.5942393340342202No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA2178010.5680636647778332No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAG2110070.5503437069332843No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1852540.48317531211864373No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1788280.46641516358919544No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1461330.3811408006619763No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1293540.33737819061286145No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTA1135440.29614290454834596No Hit
CTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCG1065140.277807416816939No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACC1061390.2768293502594315No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC829230.21627790172851485No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA807410.21058685845256464No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA784850.20470281004259963No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT778080.2029370738841128No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCA768370.20040453354454005No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAA763220.19906132213889643No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC715940.1867298589818421No Hit
TTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATATCTCG715820.18669856085200187No Hit
AGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATATCTCGTATGC691790.1804311103514939No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT668480.17435144863002738No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT639260.16673035401392908No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC639080.1666834068191687No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT638470.16652430799248083No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT625370.16310759548492137No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG623790.16269550344202488No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT620710.16189218477612538No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGC567750.14807927680663302No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA565720.14754981677683565No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT557890.14550761380476002No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA547350.14275859473379232No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA525810.13714058042746932No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA509870.13298314551369084No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC509030.13276405860480917No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT502030.13093833436412855No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCTGTAGG492600.12847882299418306No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT479660.1251038413264106No Hit
TCACCCCTCCTCCCCGCGCCCCCGCCCCGGCGACGGGGGGGGTGCTGTAG477360.12450396050447264No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC473890.12359892291659239No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC455130.11870598195156828No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG437050.11399039705563888No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT435710.11364090127242286No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC429150.11192993684115643No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA429070.11190907142126294No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC427520.11150480391082652No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTA423360.11041980207636488No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT412870.1076838238928306No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA412310.10753776595357617No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA410520.10707090218345924No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA410340.1070239549886989No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACCTGTAGGCACCATCAATAT404990.10562858003332157No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCGACGGGGGGGGTGCTGTAGGC400960.10457748450618685No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA389370.10155460679911704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)