FastQCFastQC Report
Sun 2 Jul 2023
SRR3623938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43031308
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA17127793.9803089415734236No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT14203973.3008455146192626No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8189921.9032468174102448No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3156430.7335194180014235No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3146070.7311118685957675No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3088580.7177518285058869Illumina PCR Primer Index 6 (96% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2886190.6707186311882501No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA2627040.6104950377060349No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT2565000.5960776279447513No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2510380.5833845441091403No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2498800.580693480198185No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2147410.49903433100383565No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1828470.4249162028725689No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1511450.3512442615037405No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1505050.34975697229561326No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1447940.33648524000246516No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1267870.2946389637981722No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1219660.28343549306007615No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1097100.25495390472443924No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT1076210.2500992997935364No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1033200.24010425153704368No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATATCT1026910.2386425251121811No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT1011740.235117184911042No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC986740.22930746144179487No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA912670.21209441274710963No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC911270.2117690682328318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATATCT895800.20817401135006167No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT868040.20172289440980973No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC865660.2011698087355374No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA847720.19700075117400567No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA807420.18763547694157937No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA782440.18183040125110767No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC774380.17995734640462244No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA744000.17289737044479336No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC742460.17253949147908776No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA735400.17089882557137237No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA725940.16870042621060927No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA694070.1612941907320131No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATAT693640.16119426348834204No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC677560.1574574493529223No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC668830.15542869391746122No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT666560.1549011710264536No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCTGTAGGCACCATCAATA658740.1530838895252731No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT637810.14821998903681943No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATC626010.1454777995593348No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAA594130.13806924019135092No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT590590.13724658334810552No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAA586340.1362589303583335No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCATC576480.13396757542206245No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATAT575180.1336654698016616No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACC562770.13078152307152735No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCTGTAGGCACCATCAATATCTC561220.13042132021643404No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA556680.12936627443441878No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACC551580.12818109084669235No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT531010.12340085037619586No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAATAT523180.12158124498562767No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA485820.11289919423318481No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATATC480570.11167915230464293No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAT471230.10950863961653222No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA468820.1089485822740968No Hit
GAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATATCT462130.10739390027372628No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA457560.10633188282354791No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAT441170.10252302811710953No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCTGTAGGCACCATCAATA436570.10145403899876806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)