FastQCFastQC Report
Sun 2 Jul 2023
SRR3623939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3623939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37265940
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA14092023.781474450932943No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT11917943.1980784598483227No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7845442.105257508599005No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT6153041.6511162740024805No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2622840.7038169438366508Illumina PCR Primer Index 6 (96% over 29bp)
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC2400240.6440841154147728No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2320390.6226570428654155No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2273990.6102059950721759No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA1821980.4889129322915241No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG1717420.46085514010917206No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1507930.40464026937197883No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG1443500.38735102348149547No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1406830.3775109389431744No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1299410.3486856899356356No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1139770.3058476453297569No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1114610.2990961720004916No Hit
GAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATATCT1098280.2947141545336036No Hit
CAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT1008730.27068416897574565No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA988060.2651375491937142No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATATCT961630.2580452820994184No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT928670.24920074470146197No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA916140.24583842511419277No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAATAT895100.24019251895967203No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT891610.23925600695970636No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA862950.23156533821500278No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT858880.23047318811762163No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC832990.22352582545885064No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA827880.22215460015231067No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATATCT822550.2207243397053717No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT747600.20061214073762798No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCATC664800.1783934606238297No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCTGTAGGCACCATCA633100.16988703357543108No Hit
TCCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT621510.16677695504259385No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC587480.1576452921890606No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC566990.1521469738855373No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC564130.15137951706035055No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA537660.14427651630416408No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAT532510.1428945573357334No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCTGTAGGCACCATCAATA496490.1332288948031366No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATATC479920.12878247536490425No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT472960.1269148181959183No Hit
CCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCA465310.12486200535931737No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA455110.12212492157718281No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT449460.12060879183511806No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT432410.11603356845419706No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCTGTAGGCACCATCAATA420550.11285103770359743No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTGTAGGCACCAT414840.11131880746869663No Hit
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT414580.11124903866640691No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCCTGTAGGCACCAT404100.10843681925103726No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAA393950.1057131525462661No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGTAGGCACCATCAA385830.10353421918244916No Hit
TAGGCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT384840.10326856105065377No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGCTGTAGGCACCATCAAT381080.10225959683292574No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAT377550.10131235117106935No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)