FastQCFastQC Report
Mon 25 Sept 2023
SRR364363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR364363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20151889
Total Bases866.5 Mbp
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC19858739.854525300332886No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC12321806.114464008808305No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAAT2570541.2755826513335797No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGT2469281.225334260227416No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC2161661.0726835583502867No Hit
AAGGCTGAGGCGTGATGACGAGGCACTCTGTAGGCACCATCAA2100991.0425771995865996No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC1974040.9795806239305904No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACCA1842550.9143311577391082No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC1508550.7485898716492533No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCA1494740.7417369160776938No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGC1155850.5735690584639485No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC1151230.5712764694168373No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC1143600.5674902238693355No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGG1021660.5069797675046741No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCAC947940.4703975890299912No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCA757360.37582580967967816No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACC661900.3284555606672903No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA640830.31799996516455603Illumina Single End Adapter 1 (95% over 22bp)
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGCTGTA573270.28447457208602134No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTG531530.26376187363874426No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTG512970.2545518189386613No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACC490570.2434362356799405No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCA456910.22673308690813054No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATC435040.2158805062890134No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCA423990.2103971493689748No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCA415850.2063578258097789No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCA376560.18686089428142444No Hit
AAGGCTGAGGCGTGATGACGAGGCACTACGGTGCCTGTAGGCA354930.17612740919722214No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCTGTAGGCACCAT341730.16957715477690455No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACC305490.15159372900475981No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGC277370.13763970216390137No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCA251940.12502053777688038No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCAT248630.12337801185784618No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCA236610.11741331048419333No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACC224300.11130470200585166No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAG219270.10880865808659426No Hit
TTCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCAC211940.10517128195773606No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCCTGTAGGCA208900.1036627385154811No Hit
TGGTATCCTGTCTGAATATGGGGGGACCTGTAGGCACCATCAA208320.10337492430610351No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGC205100.10177705921266239No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACACCTGT202780.10062580237515202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)