FastQCFastQC Report
Mon 25 Sept 2023
SRR364364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR364364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19274111
Total Bases828.7 Mbp
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC6659943.4553811586952055No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC4605892.389677012859374No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGT3546051.839799511375648No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACCA3008001.5606426672545364No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACC2078711.0784985102555444No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAAT1353890.7024396611599881No Hit
AAGGCTGAGGCGTGATGACGAGGCACTCTGTAGGCACCATCAA1264340.6559783743073805No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCA1231100.6387324427051396No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCA1074510.5574887474706356No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGC1017090.5276974901721796No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC984900.5109963307775908No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTG900770.4673471061778154No Hit
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATC881710.4574581935322465No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACC858240.445281237614539No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCA774270.40171502592259645No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGG731590.3795713327582268No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCA731430.3794883198503941No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGG726080.3767125757447386No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC702270.3643592173978867No Hit
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGCTGTA691920.3589893199224597No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACC680420.3530227671719852No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTG648470.33644612713914535No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCA615660.3194232927267047No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCA600000.3112984043725804No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCA568440.29492410830258264No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC552350.286576122758658No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA510110.26466071509082834Illumina Single End Adapter 1 (95% over 22bp)
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCTGTAGGCACCAT503330.2611430431214182No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCA476870.24741478348858734No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACC472610.24520456481754205No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACC441020.2288147038273257No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGC430600.22340848820472187No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCAC398270.20663469251577934No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC395880.20539468720502854No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCA393790.20431033109646404No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCAT391820.20328823466877408No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACC379100.19668870849607537No Hit
AGGTAGCGCCTCGTGAATTCATCTCCGGGCTGTAGGCACCATC371630.19281304336163677No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATC352430.18285149442171417No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC352160.1827114101397465No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCA332480.1725008224763259No Hit
GCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGT317510.16473392728723002No Hit
ACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTA307650.15961825684204062No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCCTGTAGGCA306440.1589904717265559No Hit
TGGTATCCTGTCTGAATATGGGGGGACCTGTAGGCACCATCAA304650.1580617648201777No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCAC298500.15487095617535876No Hit
ACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGCTGTA292010.15150374510139533No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCCTGTAGGCACC286270.14852565703289763No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACACCTGT284880.14780448239610117No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGCTGTA281060.14582254922159574No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCCTGTAGGCACCATCAA280380.1454697443633068No Hit
AGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACC273960.14213885143652022No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAA265460.13772879070790867No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGCTGTAGGCACCA264310.1371321354328612No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGC253620.13158583552828973No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCA253190.13136273833848938No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGT253120.1313264201913126No Hit
ATGCAAGTTTCAGTTGAAACCACTCAAGGCCCTGTAGGCACCA246360.12781912483538152No Hit
TGCAAGTTTCAGTTGAAACCACTCAAGGCCCTGTAGGCACCAT242540.1258371916608761No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATC234770.12180587732425116No Hit
ATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGT233140.12096018332570566No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAA230950.11982394414974573No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAAT226880.11771230330675174No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCAT226780.1176604202393563No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCA224380.11641522662186599No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCC223710.11606761007031662No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGC217950.11307914538833982No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCAC211420.10969118108741825No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCA206000.10687911883458595No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACC204880.10629802847975713No Hit
AGTGGCCCGTATAGCAGGCATTAACATTCCTGCTGTAGGCACC201060.1043160953052517No Hit
AGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGT199060.1032784339573431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)