FastQCFastQC Report
Mon 25 Sept 2023
SRR364365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR364365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19888911
Total Bases855.2 Mbp
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC11654825.859958848425638No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC5776322.9042917432734248No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAAT2867621.4418185088162947No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGT2706831.3609744646149808No Hit
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGCTGTA2529471.27179914475961No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACCA1953860.9823866173467215No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCC1473430.7408299026527898No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGC1424160.716057304494952No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCA1365310.6864679519155171No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGG1001440.5035167586601398No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC994640.5000977680477328No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTG980040.49275699408579987No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC942230.47374640069534224No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC828960.41679506736190836No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTG749670.37692863123576753No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCA744760.37445991889651475No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC740680.37240852452907053No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCAC699520.3517135754692653No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC559500.28131253641790643No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACC531300.2671337812311594No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA493710.2482338022428679Illumina Single End Adapter 1 (95% over 22bp)
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGC433470.21794556775883805No Hit
GCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGT382820.19247911562377648No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCA382390.19226291474681545No Hit
AAGGCTGAGGCGTGATGACGAGGCACTACGGTGCCTGTAGGCA381200.1916645913896442No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACC371380.18672716671113868No Hit
AAGGCTGAGGCGTGATGACGAGGCACTCTGTAGGCACCATCAA343280.17259869079810353No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCTGTAGGCACCAT342140.17202550707778821No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACC321940.16186909378799072No Hit
TAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCT305870.1537892145024934No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCA277790.1396707944442006No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACC260310.1308819773993659No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCCTGTAGGCA245410.12339036561629743No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCA239800.1205696983610616No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAG238320.11982556511012593No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACACCTGT224800.11302780730428126No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCA218120.10966915182032842No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCT212460.10682334492823665No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCAC210250.10571217297920435No Hit
AAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGCTGTAGG209400.10528479915265344No Hit
TCGGGAGAAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGG208200.10468144786811102No Hit
TCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCCTGTA207330.10424401818681778No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGG202090.10160938424431584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)