FastQCFastQC Report
Mon 25 Sept 2023
SRR364366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR364366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19279468
Total Bases829 Mbp
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC3724791.9319983310742805No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACC3501811.816341612745746No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACCA2878601.4930909919298603No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAAT2793621.449013012184776No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGT2758201.430641135948357No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC2525711.310051708895702No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGC1678540.8706360569700367No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGG1320410.6848788566157531No Hit
AAGGCTGAGGCGTGATGACGAGGCACTCTGTAGGCACCATCAA1175650.6097937972147364No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCCTGTAGGCACCA924790.4796760989462987No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACC831770.43142787964896123No Hit
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGCTGTA831650.4313656372675843No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCA676020.35064245548684225No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACC648890.33657049043054504No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTG645480.33480176942641776No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTAGG635250.3294956064140359No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA615650.31932935078914004Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGTTAAGTTGCAGGGTATAGACCCGCTGTAGGCACCATC539440.2798002517496852No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC538080.2790948380940802No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGC530440.2751320731464167No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCA507060.2630051825081481No Hit
GCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCA488240.2532435023622021No Hit
TACGGTGCTGAAGCAACAAATGCCCTGCTTCTCTGTAGGCACC478900.24839897034503233No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCA473880.24579516405743143No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACC440670.22856958501137065No Hit
ACGGTGCTGAAGCAACAAATGCCCTGCTTCCTGTAGGCACCAT440190.22832061548586302No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCA429850.22295739695721895No Hit
GCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACCA419010.21733483517283775No Hit
AGGTAGCGCCTCGTGAATTCATCTCCGGGCTGTAGGCACCATC401440.20822151316623466No Hit
ATGCAAGTTTCAGTTGAAACCACTCAAGGCCCTGTAGGCACCA394820.20478780846027492No Hit
GCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGT392940.2038126778187033No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC379160.19666517769058772No Hit
TGCAAGTTTCAGTTGAAACCACTCAAGGCCCTGTAGGCACCAT376460.19526472410960719No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATC375960.19500538085387004No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTG372820.1933767052078408No Hit
GCGGGAGTGGCGAAATTGGTAGACGCACCAGCTGTAGGCACCA361430.18746886584214875No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACC357250.18530075622418626No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCAC353000.18309633855042057No Hit
AGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGCTGTA317170.16451180084429715No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCA309760.16066833379427276No Hit
AGCCGATATAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACC305690.15855727969257244No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCA301060.15615576114444651No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAG298560.1548590448657608No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC297210.15415881807527054No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCCTGTAGGCA294550.15277911195474897No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGT276880.14361392129699843No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCAC268090.13905466686113954No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCAT266910.1384426167775999No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGCTGTAGGC264730.13731188018258597No Hit
ACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGCTGTA263880.1368709966478328No Hit
AGCCGATACAGCTCAGTTGGTAGAGCAGCGCACTGTAGGCACC256040.13280449439787445No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCC253510.13149221752384455No Hit
ACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGCTGTA249640.12948490072443908No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCA243910.12651282701369146No Hit
AGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCAC238820.12387271267028736No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC236770.12280940532176511No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACACCTGT227460.11798043389993956No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGCTGTAGGCA222350.11532994582630599No Hit
AGTGGCCCGTATAGCAGGCATTAACATTCCTGCTGTAGGCACC218760.11346786125011335No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACC216150.11211408945516546No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAA215490.11177175635759246No Hit
AGGTAGCGCCTCGTGAATTCATCTCCGGGGCTGTAGGCACCAT214610.11131531222749508No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA214490.11125306984611814Illumina Single End Adapter 1 (95% over 22bp)
ACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACCATC209210.10851440506553396No Hit
ATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGT208650.10822394061910837No Hit
TCGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAG202250.10490434694567297No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC201560.10454645325275573No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCCTGTAGGCACC199480.10346758530888923No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAA199190.10331716622056168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)