FastQCFastQC Report
Mon 25 Sept 2023
SRR364367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR364367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24137567
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC7447433.0854103895392604No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA2556371.0590835439213904Illumina Single End Adapter 1 (95% over 22bp)
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC1769360.7330316265926885No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTG1380950.5721164854767674No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCT1343700.5566841098773543No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTGTAGGCACCATCAAT1310800.5430539043143826No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAAT1208090.5005019768562424No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC1158220.47984123669133677No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAG1082210.44835090462928595No Hit
TAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTG1057920.43828775286258137No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTG1056760.437807174186197No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC921650.3818321871462853No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC879780.3644857826805825No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGC815230.3377432365076397No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC719520.29809135278630194No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCCTGTAGGCACCATCAA711770.29488059007769923No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTCTGTAGG689420.2856211647180513No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAC683660.2832348430146253No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGG629350.2607346465366621No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCAT604710.25052649258311743No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC578730.23976318739995628No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACT558510.23138620392022113No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAG517470.21438366178331064No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTCTGTAGGCA512580.2123577740871729No Hit
TTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCACTG510370.2114421888502681No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCA486000.20134589372657152No Hit
AGGTAACATCAAATCGTACCCCAAACCGACCTGTAGGCACCAT459940.19054944518641834No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTG448030.18561522791423013No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAA431570.17879598221312032Illumina Single End Adapter 1 (95% over 22bp)
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAG420410.17417248391273238No Hit
TGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACC397060.16449876659068416No Hit
AAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTGT393800.163148174793259No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCAC390580.16181415467432986No Hit
ATCAGGTAACATCAAATCGTACCCCAAACCGACCTGTAGGCAC380490.1576339487737103No Hit
AGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTGTA375440.1555417743635885No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCA372700.15440661438661155No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCAC351690.14570234025658013No Hit
AGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGCTGTA340130.14091312517123206No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGT334630.1386345193780301No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGT331760.1374455014459411No Hit
AGTCGATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTA310580.1286707976823016No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCCTGTAGGC295120.12226584394359216No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCA291030.12057138981737471No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCTGTAGGCA290300.12026895668482247No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCA281490.11661904449607535No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCA278600.11542174072473833No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGT268960.11142796620719894No Hit
ATAACGGTCCTAAGGTAGCGAAATTCTGTAGGCACCATCAATT268620.1112871069399828No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACC268200.11111310431577467No Hit
TTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCT264030.10938550683256519No Hit
AGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTGTAGGCA249030.1031711273965599No Hit
ACTAACTGGAGGACCGAACCGACTAATGTTGCTGTAGGCACCA248000.10274440667528753No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCA247820.10266983412205545No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATC247300.10245440230160728No Hit

[OK]Adapter Content

Adapter graph