FastQCFastQC Report
Mon 25 Sept 2023
SRR364369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR364369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23384961
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC17691097.565156939966673No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC3219811.3768720845846183No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAAT2432621.0402497570981624No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC2142670.9162598133047987No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCT2102220.8989623715857384No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC2057260.879736339949423No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC1749470.7481175615388027No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC1443030.6170760772275823No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAG1249830.5344588772245547No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACC1087660.4651108890025517No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTG1087490.4650381927085532No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA1010110.4319485501814606Illumina Single End Adapter 1 (95% over 22bp)
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGT953790.4078646956050087No Hit
AGGTAACATCAAATCGTACCCCAAACCGACCTGTAGGCACCAT797930.34121502276612736No Hit
AGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTGTA744040.31817029756859544No Hit
TAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTG705870.30184784143963295No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTGTAGGCACCATCAAT698730.2987945970916949No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACC659930.2822027370496791No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTG630190.26948516185252563No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACCTGTAG601610.2572636319555975No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCA572170.24467434433608848No Hit
ATGCGTTGTTGGGTAGGGGAGCGTTCTGTCTGTAGGCACCATC541930.23174295650952764No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCA531510.227287101312677No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCA529960.22662428216151395No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATC495200.21176002816511003No Hit
ATCAGGTAACATCAAATCGTACCCCAAACCGACCTGTAGGCAC491430.2101478809393781No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCA483540.206773917647329No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTCTGTAGG481070.2057176832580563No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCAT460660.19698985172564537No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAC453070.19374417601124072No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGG442160.18907878443757078No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC436950.18685085683914546No Hit
AGAAGTGAAACGCCGTAGCGCCGATGGTCTGTAGGCACCATCA433200.1852472621185898No Hit
ACGTATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGC417160.17838815296719973No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCCTGTAGGCACCATCAA404680.1730513897371905No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGT403500.17254679193178898No Hit
ACTAACTGGAGGACCGAACCGACTAATGTTGCTGTAGGCACCA381930.1633229150991528No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTG379740.16238641578234833No Hit
TTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCACTG355530.15203360826644097No Hit
TTGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGT345670.14781722321452664No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCAC337100.14415247474648343No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCA336000.1436820869617871No Hit
TACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAG330210.14120613671324916No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAA330070.1411462691770151No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGT310370.1327220515783627No Hit
AGTGAAATTGAACTCGCTGTGAAGATGCCTGTAGGCACCATCA309290.13226021629884266No Hit
GACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC305940.13082767168181292No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAA304970.13041287518076253No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACCTGTAGGCACC298360.12758627221999644No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCAC298240.12753495718893865No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTC294880.12609813631932076No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCACTGTAGGCA293800.12563630103980075No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTCTGTAGGCA285460.12206990638128495No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGAAACCTGTAGGCA281380.1203251953253204No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGT268250.11471047567708152No Hit
TGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACC266510.11396640772674368No Hit
AAGGCTGAGGCGTGATGACGAGGCACTACGGTGCCTGTAGGCA260950.11158881128773318No Hit
AGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCCTGTAGGCA258850.11069079824422201No Hit
TTAGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCAT258650.11060527319245904No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCA255590.1092967399004856No Hit
ATCAGGTAACATCAAATCGTACCCCCTGTAGGCACCATCAATT254830.10897174470378633No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATC251800.10767604016957737No Hit
CACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCAC249900.10686355217782917No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACT243670.10419944681541268No Hit
TATGCGTTGTTGGGTAGGGGAGCGTTCTGTCTGTAGGCACCAT240100.1026728246414437No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCAC237860.10171494406169845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)