FastQCFastQC Report
Mon 14 Aug 2023
SRR3666772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3666772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92376360
Total Bases9.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA44554174.823113835617684No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC37968464.110192261310145No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA30960143.351521969473575No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT17015721.841999403310544No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA13036371.411223607425103Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA12054291.3049106936016963Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC11000701.1908566217590735No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG10774991.1664228813518955No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT10623791.1500550573761512No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC9714931.0516684138669246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC9675491.0473989232743095Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT8439350.913583302048273Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA7751290.8390988776782284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC7690150.832480301237243No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC7011780.7590448465386599No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG6483740.7018830358762783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6125420.6630938911210617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC5408270.5854603926805516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA5075550.549442519709588Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4973540.5383996511661642No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG4336230.4694090566027932Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4020840.435267204726404No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC4007720.4338469279369744Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3830680.4146818515039995No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC3808780.41231111509481433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3706670.4012574212709832No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACAC3705670.4011491684669108No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC3586730.38827357995054146No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC3447450.3731961293993398Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT3335870.36111728152094325No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT3262540.3531791033983153No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAGATCGGAAGAGCACACG3073510.3327160758445126No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA2921920.3163060332751799No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTGAA2724360.2949196093026398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2723280.2948026962742416TruSeq Adapter, Index 6 (100% over 49bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC2678330.2899367327311879No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2644130.28623448683191244No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2519960.2727927361502445No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT2453010.26554521091759836Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG2452440.2654835068192771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2430730.2631333384428657No Hit
ACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTC2429880.2630413235594041Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCCGCCGGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTC2207310.23894749695701367No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2165960.23447124350862059No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTCTG2121240.2296301781105036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2104560.22782452133857625No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1961620.21235086552446966No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC1876420.20312772661750259No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC1874620.20293287157017226No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1863290.20170636730003216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACG1816680.19666070410221834No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTC1763750.1909308831826671No Hit
GCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGT1650500.17867125312146961No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTG1628720.17631350704877308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT1614910.17481853582453347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG1560760.16895664648401387No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACAC1539760.16668333759849382No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCT1522310.16479432616743073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1492650.1615835479986438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTCT1472040.15935245770671197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1452150.15719930943371227No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTCTGA1418620.15356959291316524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1406220.15222725814266766No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1349490.14608607656764133No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA1300920.14082823787384566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA1277650.1383091951230813No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA1251510.13547946682462916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1244290.13469788157922655No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1197240.12960458714762088Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA1193120.12915858559484267No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1157190.12526906234452193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC1129360.12225638680718746Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCC1119910.12123339780870344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1114030.12059687132075783No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1093380.11836145091666309Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1088890.11787539582637808No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG1073240.11618123944264527No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1058460.11458126299845545No Hit
GACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCT1041650.11276153336199866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTC1033420.11187061278448295No Hit
GGTGAAATACCACTACTCTCATAGATCGGAAGAGCACACGTCTGAACTCC1032110.11172880161114815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA1000100.10826362935279113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC993580.10755782107023917No Hit
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAA988810.10704145519481391Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT984830.10661060903460581Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT963280.10427776110684595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGA932400.10093491451709073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG925850.10022585865041662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)