FastQCFastQC Report
Mon 14 Aug 2023
SRR3666773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3666773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103144951
Total Bases10.4 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA42856224.154950832251595Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA17903461.7357572839411208Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC12482501.2101901139106652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC10711891.038527809276869No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC10320021.0005356442507787Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA9753420.9456032414034498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC7926540.768485507351688No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA6710170.6505572919415125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA6673760.647027308200476No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG5995800.581298448626923No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5845710.5667470819778663No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG5731070.5556326261670336TruSeq Adapter, Index 12 (100% over 49bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG4982380.4830464265769054No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4825400.4678270679482896No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC4454330.43185148248313193No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4386950.4253189281169953No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG3980770.38593939513335945No Hit
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC3719930.3606507118317405Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG3638640.3527695698842302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA3466380.3360688008858524No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC3297990.31974323202693655No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT3095000.30006316062916155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3029510.2937138435404366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA2847230.27604162611895566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2791550.2706433977558436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA2597720.25185139697240244No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA2589850.25108839307122266No Hit
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAACT2455840.23809599754427146Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2406770.23333861489739813No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGA2281460.2211896925521832Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC2272420.22031325605070093Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2145300.20798885250330867No Hit
ACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT2129920.20649774703950366Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG2112670.20482534331709557No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACAC2069610.20065063582220327No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1994330.19335216902667393No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC1986890.19263085402987878No Hit
GACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACT1924180.1865510605555477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC1916790.18583459310577402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1725940.16733150612481265No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGT1725360.16727527457936356No Hit
GACCTGGTATTGCAGTACCTCCAGAGATCGGAAGAGCACACGTCTGAACT1703900.16519470739774747Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1671520.16205543594664173No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1654530.1604082394687453No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG1648170.15979163148761397No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG1609180.15601151432026952Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC1596060.1547395179818351No Hit
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACT1533530.14867717567678132Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1509840.14638040789800752No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1453790.1409463076869366No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA1420900.13775759125621184No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG1371970.13301378174099865No Hit
ATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTCTGAACTC1302960.12632319734196198Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1263870.12253338508057462No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA1244310.1206370246857745Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1242690.12047996416227878Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1218210.11810660514056573No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1187190.11509918696844404No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGA1174750.11389311726950165No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1169510.1133850943416513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1107070.10733147762123617No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1092400.10590920732513606No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1087560.1054399647734575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG1083340.1050308318048452No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1055170.10229972381294748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT1052910.10208061468757691Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG1051130.10190804201361248No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1045230.1013360314650787No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1034650.10031029051533506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)