FastQCFastQC Report
Sun 2 Jul 2023
SRR3726644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3726644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50902331
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC5537121.0877930128582913No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA4087080.8029259013698213No Hit
AAATACCACTACTCTCATCTGTAGGCACCATCAATAGATC3492710.6861591466213993No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTG3366690.6614019306895788No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3117250.6123982809353072No Hit
GTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG3109430.61086200551405No Hit
TGAAATACCACTACTCTCATCTGTAGGCACCATCAATAGA2844750.558864386780244No Hit
CCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG2798120.549703706103361No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCA2694130.529274386275159No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC2387120.46896084189150394No Hit
CCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCAT2294780.4508202188225919No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA2216010.43534548545527313No Hit
TCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2215690.43528261996488926No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC2008820.39464204497825456No Hit
TAATTCTAGAGCTAATACATGCCTGTAGGCACCATCAATA1915050.3762204917491892No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCA1794800.35259681919085395No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATC1768060.347343621650647No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCAT1752520.3442907162738775No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1725010.3388862486474342No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGC1648570.3238692546319735No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCT1626590.3195511812612275No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCG1614790.3172330163033202No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA1527860.30015521293121133No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC1486670.29206324559085517No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCA1450980.2850517788664727No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCA1418560.27868271887195106No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACC1408400.2766867395522614No Hit
CCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAA1339480.263147084560823No Hit
AAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCA1273530.2501908999020104No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCC1266180.24874695816975456No Hit
AAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCA1239830.243570377945953No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCAT1225170.24069035266773933No Hit
GTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCAAT1203880.23650783301063363No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG1180060.23182828307018005No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCAC1148330.2255947767892987No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGC1144410.22482467453209556No Hit
CCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1115010.21904890760307225No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1092550.2146365359967503No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACC1021260.20063128346715595No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCT1016350.19966669109907756No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCAT980580.19263950800209917No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCC956780.18796388715479453No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTG941530.18496795362868548No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATC929220.18254959679547877No Hit
GCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCA899890.1767875816924769No Hit
AGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA896310.17608427401880672No Hit
TAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAAT881730.17321996511318902No Hit
GAAATACCACTACTCTCATCTGTAGGCACCATCAATAGAT848310.1666544504612176No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCAC823320.16174504857154773No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA818940.16088457717191773No Hit
TGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCA808620.15885716510703607No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATC804050.1579593673224906No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCAT783280.1538790040872588No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACC770050.15127990896919827No Hit
GATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGG747060.14676341639442797No Hit
CCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCA745090.14637640071925193No Hit
GTGAAATACCACTACTCTCACTGTAGGCACCATCAATAGA719440.14133733875566523No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGC710360.13955353046602129No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACC708750.13923723846752714No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCA692510.13604681483054284No Hit
AGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCAT690960.13574231011149568No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACC686100.13478754047628977No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACC678950.1333828896755239No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC645530.12681737502355245No Hit
CCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATC623890.12256609623633935No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCAC620810.12196101589139405No Hit
AAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAAT619340.12167222754494289No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACTGTAG611790.12018899488119708No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGC609450.11972929098276461No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATC592980.11649368277456686No Hit
GTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAA592500.11639938453899097No Hit
TGAAATACCACTACTCTCACTGTAGGCACCATCAATAGAT586430.11520690476827082No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGG585260.1149770528190546No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGT582510.11443680251106772No Hit
ACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATA575150.11299089623223738No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA567160.11142122351921369Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTAAAGCATCTGGCCTACACCCAGAAGATTCTGTAGGCA566020.11119726520972095No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGG560990.11020909828274858No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCAC546890.10743908761270678No Hit
AAATACCACTACTCTCACTGTAGGCACCATCAATAGATCG533250.10475944608509186No Hit
TCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGA531150.10434689130444734No Hit
CGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATAGA526640.10346088079934886No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGC526610.10345498715962535No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT525530.10324281612957961No Hit
AATACCACTACTCTCATCTGTAGGCACCATCAATAGATCG523010.10274775039280618No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG521050.10236269926420462No Hit
TTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCAAT512080.10060050098688014No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)