FastQCFastQC Report
Mon 3 Jul 2023
SRR3726646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3726646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60441316
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG4041790.6687131034671713No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATC3197030.5289477813487714No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC2918360.4828419023834623No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA2163450.35794223937811015No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCT1824860.3019226120093083No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGC1808110.29915132886914636No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT1775760.29379902978949035No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAG1720420.28464304119387474No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCA1714320.28363379778163667No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT1619400.2679293084882533No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTC1606680.2658247877991273No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1601710.26500250259276287No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG1371300.22688122806591438No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTC1361000.22517709574688943No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGG1275510.21103279749898232No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG1258320.2081887164733475No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCT1219630.20178746604392264No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCC1167190.19311128169346942No Hit
GCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATA1151830.190569973691506No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA1113600.18424483014234833No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT1092730.1807918940745764No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCA1080970.17884620513557314No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCA1064950.17619570030540035No Hit
CGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA1039140.17192544252345532No Hit
TGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTG1018830.16856515830992166No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA989670.1637406438999442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCT988310.16351563225393703No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTGT985420.16303748250617178No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGG979050.16198356766421168No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCT943170.15604723100337525No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGT942130.15587516327407563No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGC924070.1528871409748921No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTA908570.15032267000936908No Hit
CGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAAT901510.1491545948470083No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACC892280.1476274937494743No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTG848620.14040395811368503No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG847850.14027656181410741No Hit
CATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAAT842480.1393880967118585No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCC818870.13548182835727798No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTA798480.13210830816456742No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTG790180.1307350753249648No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG773220.1279290477394635No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGG745730.12338083439480373No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGC736880.12191660419835994No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG726350.12017441843920143No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTA725090.11996595176716536No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGC723110.11963836128253726No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAGGCA711950.11779194218736072No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC697390.11538299397716621No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA692740.11461365268750931No Hit
TAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAAT683820.11313784100928576No Hit
GCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCAATA683550.11309316957956377No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT673000.111347674825611No Hit
GCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC670360.11091088751277355No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCA653680.1081511858543914No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCT645880.10686067788464435No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA644150.10657444983494402No Hit
TGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC632360.10462379740374946No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTG630980.10439547676294805No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATA630630.10433756935404913No Hit
GCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAAT629400.10413406617420441No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA623450.103149640222923No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACC615310.10180287934167416No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC609500.10084161635395231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)