FastQCFastQC Report
Mon 3 Jul 2023
SRR3726648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3726648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49923346
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG6175021.2369002670614266No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGG4266560.8546222042088285No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTC4234230.8481462760929527No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGC3700420.7412203500943226No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCA3385490.6781376392519844No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT3245260.6500485764716171No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCT2988400.5985976981590937No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCT2891180.5791238431815047No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGC2890130.5789135207403767No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCT2706300.5420910689760258No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT2480020.4967655813774982No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA2453750.49150351420756133No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCA2278070.4563135652005376No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1962820.39316675609042706No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTC1936900.38797479640086624No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTA1884810.3775408002500473No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAGGCA1878760.3763289423749762No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTG1863480.3732682500888462No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG1567850.3140514660215283No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCA1450590.2905634570246954No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCA1436680.2877771854474658No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG1393080.2790437964634822No Hit
CGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAAT1331210.2666507970038707No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA1253550.25109494864386694No Hit
CGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTA1249240.250231625099808No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCT1166250.23360813996722096No Hit
GCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATA1137910.22793143712763164No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAG1137620.22787334807246293No Hit
CGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAA1119980.2243399310615118No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAG1118180.22397937830529227No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGC1041100.20853970805562594No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTG1015170.20334574529519717No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG999780.20026301922952042No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACC986490.19760093804609974No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGT974120.19512313938252454No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG955680.19142947670214253No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGG955170.19132732008788034No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA952270.19074642953619333No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC943570.18900375788113238No Hit
CCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCA939900.1882686308726182No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTA936460.18757957449406537No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCAC923750.1850336714209821No Hit
GTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCA890360.17834541779311028No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGC886210.17751414338293753No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC878230.17591569283036437No Hit
GCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGT868740.17401477857674044No Hit
GATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGG846980.16965609636822018No Hit
CGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAA843470.16895301849359215No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT833490.1669539537674418No Hit
TTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCAC814640.16317816518147643No Hit
CGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAG805560.16135937683343582No Hit
GCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAAT788260.1578940642319928No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCAC780670.15637373344326722No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCT760950.15242367769179574No Hit
CCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAAT737120.14765035981362307No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT726430.14550907705585278No Hit
CTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATA704650.14114638870559676No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGTAGGCAC699460.14010679492516387No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT692980.13880880500277365No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGT681100.1364291568117249No Hit
TTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACC672580.13472254043228593No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTC671000.13440605523515992No Hit
TCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA653000.13080052767296485No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC642010.128599152789158No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTG635630.12732119357544666No Hit
TTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA606350.12145620207427603No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC601350.12045466664033296No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCA588080.11779659159864804No Hit
CGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA586850.11755021388189806No Hit
CGCTCCCCGTTCCTCCCGACCCCCTGTAGGCACCATCAAT579770.11613203970743467No Hit
CCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATA575040.11518458718692452No Hit
CATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAAT553390.11084793875795104No Hit
GCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTA552270.1106235948207478No Hit
GATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAGGC550210.11021096222196326No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA545840.109335620252697No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATC530430.10624888804528446No Hit
TTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAGGCACC527710.10570405276921944No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT525380.10523733725700196No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACC516980.1035547577279776No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGC516630.10348465024760159No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTG511650.10248712095539429No Hit
CCGCTCCCCGTTCCTCCCGACCCCCTGTAGGCACCATCAA507560.10166786497042887No Hit
GCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAAT504890.10113304504870327No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT502270.1006082404813171No Hit
TGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACC500420.10023767237075816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)