FastQCFastQC Report
Tue 4 Jul 2023
SRR3726651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3726651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35641132
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG5353661.502101560635055No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT3133990.8793183112141332No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCT2497280.7006735925222577No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAGG2407570.6755032359802713No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTC2405630.6749589210578385No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCT2027760.5689381583054096No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT1915020.5373061663698No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGC1911360.5362792629594368No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA1865870.5235159197524928No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGC1848990.518779818777922No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGCA1833300.5143776016990705No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTA1772040.49718959543709224No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCA1644510.4614079036546875No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTC1486690.41712760414007055No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAGGCA1389910.38997358445292923No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG1389110.3897491246911013No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG1374790.38573129495438024No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1236170.34683802972363503No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCA1198410.33624352896535387No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCT1158830.325138382248914No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCA1119090.3139883435801085No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCT1099040.30836282079929445No Hit
GCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATA1090740.3060340507703291No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAG1080370.30312449110763373No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA1067900.2996257245701399No Hit
CGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAAT947980.265979206272124No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTG924870.2594951249023179No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG884640.24820760462939281No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTG871230.24444509787175112No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC852760.23926288312054733No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGC834760.23421253847941753No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTGTAG784520.2201164654366197No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGT762100.21382598061139022No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC753720.21147476460624204No Hit
CGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTA723330.20294809940380124No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCAC700280.1964808525161322No Hit
GCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAAT699000.19612171689720742No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT682260.1914248963809567No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCT665670.18677016207004873No Hit
CCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAAT647740.18173945765807886No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG618800.1736196257739513No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGG615940.17281718212541622No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTA603670.16937453052837942No Hit
CGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAA600840.16858050412091288No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACTGTAGGC597990.16778086621940067No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA594040.16667259614537494No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTG576410.1617260641440906No Hit
ATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGTAGGCAC567370.15918966883543428No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT557700.15647651146433844No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACC539880.15147667026961995No Hit
TCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA538630.1511259518917637No Hit
CGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCCTGTAG537260.15074156454963325No Hit
CTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATA535620.1502814220378859No Hit
CGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAA528320.14823322671120548No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT500460.14041641550554568No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCAC499120.1400404454044838No Hit
CCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATA498650.13990857529440984No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGT481750.13516686282579352No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTG476110.13358442150490618No Hit
GTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCA464070.1302063020893949No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCCTG460070.12908400328025496No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTC458590.12866875272087316No Hit
GATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGG445440.12497919538582557No Hit
CGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATCA441780.12395229197546252No Hit
GCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGT425670.11943223352165132No Hit
CTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATC418430.11740087267710803No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCCCCCCTG408170.11452217623166402No Hit
TTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCAC406410.11402836475564244No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC401580.11267318894360595No Hit
TTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACC399570.11210923379201311No Hit
CCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCT398580.11183146483675097No Hit
CCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCA395910.11108233038165005No Hit
CTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGAT390080.10944657986732857No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTA387980.1088573729925301No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTG381690.10709255811515751No Hit
CCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGAT380050.10663241560341012No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGT371400.10420544442864497No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)