FastQCFastQC Report
Sun 2 Jul 2023
SRR3749602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3749602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23224853
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAGGA5582342.4036061713716768RNA PCR Primer, Index 1 (100% over 22bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAG4178421.7991158006468329No Hit
TTCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGAA3768791.6227400879566385RNA PCR Primer, Index 1 (100% over 23bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGTGGAATTCTCGGGT3491611.5033937997368594No Hit
TTCCGAACCCGTCGGCTGTCAGCGGACTGGAATTCTCGGGTGCCAAGGA2900031.2486752876326064RNA PCR Primer, Index 1 (100% over 22bp)
ACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACTGGAA2760001.1883821180698109No Hit
AGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2702011.1634131763934092RNA PCR Primer, Index 1 (100% over 31bp)
TCAGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGAACTCC2496381.0748744028648964RNA PCR Primer, Index 1 (100% over 27bp)
GTTCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGA2104510.9061456707605426RNA PCR Primer, Index 1 (100% over 22bp)
ACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG2014880.8675533920494567RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGTCAGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGA1983520.854050615519504RNA PCR Primer, Index 1 (100% over 22bp)
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCTGGAATTCTCGGG1720650.7408658302379783No Hit
TTCCGAACCCGTCGGCTGTCAGCTGGAATTCTCGGGTGCCAAGGAACTC1716050.7388851933745286RNA PCR Primer, Index 1 (100% over 26bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGCTTGGAATTCT1523030.6557759482912551No Hit
AGACCGGTAGACTTGAACCTTGTTCCTACTGGAATTCTCGGGTGCCAAG1360280.5857001549159428No Hit
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTTGGAATTCTCGGGTGCCA1155550.49754889729549634No Hit
TCAGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA1127570.4855014582869481RNA PCR Primer, Index 1 (100% over 28bp)
TTCCGAACCCGTCGGCTGTCAGCGGTGGAATTCTCGGGTGCCAAGGAAC996530.4290791420725031RNA PCR Primer, Index 1 (100% over 24bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGCTGGAATTCTC961060.4138067095623813No Hit
ACGAGGATCCATTGGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG957020.41206719370839506RNA PCR Primer, Index 1 (100% over 29bp)
AACAGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAA947430.4079379964213336No Hit
ACAGACCGGTAGACTTGAACCTTGTTCCTACTGGAATTCTCGGGTGCCA913400.39328558936411784No Hit
GTTCCGAACCCGTCGGCTGTCAGCGGACTGGAATTCTCGGGTGCCAAGG854220.3678042655426064Illumina Small RNA Adapter 2 (100% over 21bp)
TGACCGAGATCTTTGGAGATGATTCTGTACTTGGAATTCTCGGGTGCCA794450.3420689035146961No Hit
ACAGACCGGTAGACTTGAACCTTGTTCCTACATGGAATTCTCGGGTGCC736600.3171602420906604No Hit
GGCTGTCAGCGGACTGCTCGAGCTGCTTCCGCGGTGGAATTCTCGGGTG694120.2988694912299337No Hit
ATGCGGACTACCTGCACCTGGACTGGAATTCTCGGGTGCCAAGGAACTC672440.2895346635778491RNA PCR Primer, Index 1 (100% over 26bp)
TCAGACTGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAA656990.2828823071560453RNA PCR Primer, Index 1 (100% over 23bp)
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTCGGGATGGAATTCT646280.278270867850057No Hit
TGACCGAGATCTTTGGAGATGATTCTGTACTACTGGAATTCTCGGGTGC623030.26826004022501243No Hit
AGACCGGTAGACTTGAACCTTGTTCCTACATGGAATTCTCGGGTGCCAA617510.2658832759888728No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCA607480.2615646264800901No Hit
ATTGGATGATGGAAAAACCAGCTGGAATTCTCGGGTGCCAAGGAACTCC604270.2601824864079872RNA PCR Primer, Index 1 (100% over 27bp)
AGGGACGCATTTATTAGATATGGAATTCTCGGGTGCCAAGGAACTCCAG585150.25194992622773543RNA PCR Primer, Index 1 (100% over 29bp)
GTTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGTGGAATTCTCGGG571340.24600370990507456No Hit
ACAGACCGGTAGACTTGAACCTTGTTCCTGGAATTCTCGGGTGCCAAGG552770.2380079650019744Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACT543650.2340811371335698RNA PCR Primer, Index 1 (100% over 25bp)
TGACCGAGATCTTTGGAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA530190.22828562144182354RNA PCR Primer, Index 1 (100% over 28bp)
ATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTGGAATTCTCGGGT528510.22756225841343322No Hit
ATGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG523190.22527160882353053RNA PCR Primer, Index 1 (100% over 29bp)
AGACCGGTAGACTTGAACCTTGTTCCTGGAATTCTCGGGTGCCAAGGAA473300.20379031031972517RNA PCR Primer, Index 1 (100% over 23bp)
AATGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCA451170.19426172471360745RNA PCR Primer, Index 1 (100% over 28bp)
ATGGAACAATGTAGGCAAGGGAAGTCGGCATGGAATTCTCGGGTGCCAA443830.19110131719671164No Hit
ACGGGCTTGGCAGAATCAGCGGGGAATGGAATTCTCGGGTGCCAAGGAA432800.18635209445674425RNA PCR Primer, Index 1 (100% over 23bp)
AGACTGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT420790.18118090995021585RNA PCR Primer, Index 1 (100% over 25bp)
TGACCGAGATCTTTGGAGATGATTCTGTACTGGAATTCTCGGGTGCCAA419410.18058671889118091No Hit
ACCGTGCGCTGGATTATGACTGTGGAATTCTCGGGTGCCAAGGAACTCC411540.17719810756175722RNA PCR Primer, Index 1 (100% over 27bp)
GCCGCTATGGTGAAATTGGTAGACACGCTGCTCTGGAATTCTCGGGTGC387200.1667179551147213No Hit
TTCCGAACCCGTCGGCTGTCAGCGGACTTGGAATTCTCGGGTGCCAAGG382200.1645650889587977Illumina Small RNA Adapter 2 (100% over 21bp)
GTTCCGAACCCGTCGGCTGTCAGCGGACTGCTTGGAATTCTCGGGTGCC381720.16435841380782906No Hit
TTCCGAACCCGTCGGCTGTCAGCGTGGAATTCTCGGGTGCCAAGGAACT381570.16429382782315133RNA PCR Primer, Index 1 (100% over 25bp)
ACGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAAC379680.16348004441621222RNA PCR Primer, Index 1 (100% over 24bp)
ACAGACCGGTAGACTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCA374410.16121092348786878RNA PCR Primer, Index 1 (100% over 28bp)
AAAGGTCGACGCGGGCTCTGCCCGTTGCTGGAATTCTCGGGTGCCAAGG369030.15889443950409504Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGGAATTCTCGGG362680.1561602994860721No Hit
TGACCGAGATCTTTGGAGATGATTCTGTGGAATTCTCGGGTGCCAAGGA352620.1518287327803539RNA PCR Primer, Index 1 (100% over 22bp)
AGACCGGTAGACTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT351990.1515574716447075RNA PCR Primer, Index 1 (100% over 30bp)
AGTTGGGGGCTCGAAGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC347370.1495682233166341RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCAGCGGACTGCTCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG336610.1449352553490866RNA PCR Primer, Index 1 (100% over 29bp)
ATAGGACTCCGCTGGCACCTTGGAATTCTCGGGTGCCAAGGAACTCCAG335070.14427217257306213RNA PCR Primer, Index 1 (100% over 29bp)
ACACGGACCAAGGAGTCTGACATGTGTGGAATTCTCGGGTGCCAAGGAA334320.1439492426496736RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGGGTGCCAAG330990.1425154337898285No Hit
AGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTGGAATTCTCGGGTGCC328450.14142177778261933No Hit
AATGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC326650.14064674596648685RNA PCR Primer, Index 1 (100% over 26bp)
TCCGAACCCGTCGGCTGTCAGCGGACTGGAATTCTCGGGTGCCAAGGAA321100.1382570645334117RNA PCR Primer, Index 1 (100% over 23bp)
TCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGTGGAATTCTCGGGTG319010.13735716648023563No Hit
GGCCGGGGGACGGACTGGGAACGGCTCTCTCGGGATGGAATTCTCGGGT316970.13647879708861882No Hit
ACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAGGAAC316650.13634101365463971RNA PCR Primer, Index 1 (100% over 24bp)
AGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC315470.13583293724184176RNA PCR Primer, Index 1 (100% over 27bp)
AGCATCGGCTAACTCTGTGCCAGCTGGAATTCTCGGGTGCCAAGGAACT315360.13578557418641143RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGACGGACTGGGAACGGCTCTCTCGGGATGGAATTCTCGGGTGCCA303890.13084689922472276No Hit
AGCCATCGTCACTGGTAAGGGACCGATAGAGAACCTTGCTGACCTGGAA301230.12970157442977143No Hit
AGAGATCGGAAAGAACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT297130.12793622418191408RNA PCR Primer, Index 1 (100% over 30bp)
TTCCGAACCCGTCGGCTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG291950.12570585484437727RNA PCR Primer, Index 1 (100% over 29bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGTGGAATTCTCG287750.12389744727340148No Hit
AGTAAACCCGTAAGGCGCAAGGAAGCTGTGGAATTCTCGGGTGCCAAGG282690.12171874672360682Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTGGAATTCTCGGGTGCCAA281190.12107288687682974No Hit
CTGTCAGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGAAC271660.11696952398363941RNA PCR Primer, Index 1 (100% over 24bp)
ATGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCC261120.11243128212695254RNA PCR Primer, Index 1 (100% over 27bp)
AAGGCAACAAATTAGAGTCTCTGATTCACTTGGAATTCTCGGGTGCCAA257720.11096733314092451No Hit
TTGGCGGCGGAGAAAGACTCGGCATGGAATTCTCGGGTGCCAAGGAACT255570.11004160069387736RNA PCR Primer, Index 1 (100% over 25bp)
ACAACGGCGGTCCTTATGAACTGGAATTCTCGGGTGCCAAGGAACTCCA251840.10843556254155838RNA PCR Primer, Index 1 (100% over 28bp)
TGTTATTAATACTTGGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG250260.10775525683628655RNA PCR Primer, Index 1 (100% over 29bp)
ATGTAGGCAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA248500.10699744794940144RNA PCR Primer, Index 1 (100% over 28bp)
TCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGAAC246520.1061449129516557RNA PCR Primer, Index 1 (100% over 24bp)
ATAGGACTCCGCTGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCA246110.10596837792686997RNA PCR Primer, Index 1 (100% over 28bp)
GTTCCGAACCCGTCGGCTGTCAGCTGGAATTCTCGGGTGCCAAGGAACT240160.10340646720132093RNA PCR Primer, Index 1 (100% over 25bp)
TCCGAACCCGTCGGCTGTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC237870.10242045450190793RNA PCR Primer, Index 1 (100% over 27bp)
AAGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAACTC237510.10226544813868144RNA PCR Primer, Index 1 (100% over 26bp)
ACGGGCCAAGGGACAGTCTCAGGTAGACAGTTGGAATTCTCGGGTGCCA236790.10195543541222844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)