FastQCFastQC Report
Sun 2 Jul 2023
SRR3749603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3749603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20643386
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGAACTCC6685173.2384076914513926RNA PCR Primer, Index 1 (100% over 27bp)
AGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAGGA5599362.7124232429699275RNA PCR Primer, Index 1 (100% over 22bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAG3912491.8952753196592846No Hit
ACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACTGGAA3344921.6203349586158007No Hit
TCAGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA2539601.2302245377768937RNA PCR Primer, Index 1 (100% over 28bp)
TCAGACTGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAA2511641.2166802480949588RNA PCR Primer, Index 1 (100% over 23bp)
AGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2429991.1771276281904528RNA PCR Primer, Index 1 (100% over 31bp)
TTCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGAA1649350.7989726104041266RNA PCR Primer, Index 1 (100% over 23bp)
ACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG1445580.7002630285554898RNA PCR Primer, Index 1 (100% over 29bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGTGGAATTCTCGGGT1434860.6950700820107709No Hit
TTCCGAACCCGTCGGCTGTCAGCGGACTGGAATTCTCGGGTGCCAAGGA1324440.6415807949335444RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGATGATGGAAAAACCAGCTGGAATTCTCGGGTGCCAAGGAACTCC1114280.5397757906575985RNA PCR Primer, Index 1 (100% over 27bp)
TCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCTGGAATTCTCGGG1112810.5390636981743209No Hit
GGCTGTCAGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGA1092020.528992675910822RNA PCR Primer, Index 1 (100% over 22bp)
ACGAGGATCCATTGGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1067130.5169355453606304RNA PCR Primer, Index 1 (100% over 29bp)
AGACTGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT865160.41909791349151737RNA PCR Primer, Index 1 (100% over 25bp)
GTTCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGA861080.4171214935379303RNA PCR Primer, Index 1 (100% over 22bp)
AGACCGGTAGACTTGAACCTTGTTCCTACTGGAATTCTCGGGTGCCAAG853890.41363853778638837No Hit
TTCCGAACCCGTCGGCTGTCAGCTGGAATTCTCGGGTGCCAAGGAACTC845400.40952584038296813RNA PCR Primer, Index 1 (100% over 26bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGCTTGGAATTCT832580.4033156188621382No Hit
AACAGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAA798600.3868551409153518No Hit
TGACCGAGATCTTTGGAGATGATTCTGTACTTGGAATTCTCGGGTGCCA667390.32329483157462635No Hit
ACAGACCGGTAGACTTGAACCTTGTTCCTACTGGAATTCTCGGGTGCCA656730.3181309500292248No Hit
TGACCGAGATCTTTGGAGATGATTCTGTACTACTGGAATTCTCGGGTGC517250.2505645149492433No Hit
AGGGACGCATTTATTAGATATGGAATTCTCGGGTGCCAAGGAACTCCAG515730.24982820163320105RNA PCR Primer, Index 1 (100% over 29bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTGGAATTCTCGGGTGCCAAGG508570.24635977838131784Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGAACCCGTCGGCTGTCAGCGGTGGAATTCTCGGGTGCCAAGGAAC491740.2382070460727712RNA PCR Primer, Index 1 (100% over 24bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGGGTGCCAAG490350.23753370692191678No Hit
TGACCGAGATCTTTGGAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA489670.23720430359631894RNA PCR Primer, Index 1 (100% over 28bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTTGGAATTCTCGGGTGCCA487100.23595935279222122No Hit
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGCTGGAATTCTC484580.234738622820888No Hit
TCAGACTGTGAAACTGCGAATGGCTCATTAAATCTGGAATTCTCGGGTG458940.22231817977922808No Hit
AGACCGGTAGACTTGAACCTTGTTCCTGGAATTCTCGGGTGCCAAGGAA456360.2210683848085774RNA PCR Primer, Index 1 (100% over 23bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTACATGGAATTCTCGGGTGCC452550.21922275735191893No Hit
ATGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG422430.20463212769455552RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAGGAAC414030.20056302779011156RNA PCR Primer, Index 1 (100% over 24bp)
AGACTGTGAAACTGCGAATGGCTCATTTGGAATTCTCGGGTGCCAAGGA393420.19057920052456512RNA PCR Primer, Index 1 (100% over 22bp)
ATGCGGACTACCTGCACCTGGACTGGAATTCTCGGGTGCCAAGGAACTC393170.19045809636074237RNA PCR Primer, Index 1 (100% over 26bp)
AATGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCA386480.1872173489368459RNA PCR Primer, Index 1 (100% over 28bp)
ACCGTGCGCTGGATTATGACTGTGGAATTCTCGGGTGCCAAGGAACTCC384570.18629211312524022RNA PCR Primer, Index 1 (100% over 27bp)
GCTACATGTGGCTTAGAACATGTGGAATTCTCGGGTGCCAAGGAACTCC372400.18039676243034936RNA PCR Primer, Index 1 (100% over 27bp)
AGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG368820.17866255080440777RNA PCR Primer, Index 1 (100% over 29bp)
GTTCCGAACCCGTCGGCTGTCAGCGGACTGGAATTCTCGGGTGCCAAGG364720.1766764425177149Illumina Small RNA Adapter 2 (100% over 21bp)
AGACCGGTAGACTTGAACCTTGTTCCTACATGGAATTCTCGGGTGCCAA363600.176133895863789No Hit
AAAGGTCGACGCGGGCTCTGCCCGTTGCTGGAATTCTCGGGTGCCAAGG360240.1745062559020114Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGCTATGGTGAAATTGGTAGACACGCTGCTCTGGAATTCTCGGGTGC359760.17427373590747178No Hit
TGTTATTAATACTTGGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG347400.16828634604807563RNA PCR Primer, Index 1 (100% over 29bp)
TGACCGAGATCTTTGGAGATGATTCTGTACTGGAATTCTCGGGTGCCAA344270.16677012191701496No Hit
ATAGGACTCCGCTGGCACCTTGGAATTCTCGGGTGCCAAGGAACTCCAG343200.16625179609585367RNA PCR Primer, Index 1 (100% over 29bp)
AGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC310920.15061482646306185RNA PCR Primer, Index 1 (100% over 27bp)
AGTTGGGGGCTCGAAGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC308230.14931174566032918RNA PCR Primer, Index 1 (100% over 27bp)
AGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACT302920.14673949322073424RNA PCR Primer, Index 1 (100% over 25bp)
TGACCGAGATCTTTGGAGATGATTCTGTGGAATTCTCGGGTGCCAAGGA292700.14178875500366075RNA PCR Primer, Index 1 (100% over 22bp)
AGTAGATCTTTTTAGGAGGACCCAATGACTATAGATAGGACCTGGAATT289400.1401901800412006No Hit
AACGAGGATCCATTGGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA281270.13625187263368518RNA PCR Primer, Index 1 (100% over 28bp)
TCAGACTGTGAAACTGCGAATGGCTCATTTGGAATTCTCGGGTGCCAAG272920.13220699356200577No Hit
ACGGGCTTGGCAGAATCAGCGGGGAATGGAATTCTCGGGTGCCAAGGAA270230.13090391275927313RNA PCR Primer, Index 1 (100% over 23bp)
TTGGCGGCGGAGAAAGACTCGGCATGGAATTCTCGGGTGCCAAGGAACT268530.1300804044452785RNA PCR Primer, Index 1 (100% over 25bp)
ATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTGGAATTCTCGGGT268060.12985272861729177No Hit
ACGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAAC265190.12846245281660673RNA PCR Primer, Index 1 (100% over 24bp)
TTGGGGGCTCGAAGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAG259590.12574971954697742RNA PCR Primer, Index 1 (100% over 29bp)
AAGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAACTC258500.1252217053927103RNA PCR Primer, Index 1 (100% over 26bp)
TTATTAATACTTGGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC256060.12403972875380037RNA PCR Primer, Index 1 (100% over 31bp)
AGCCGCGGTATCTTTTTCACTCTGGAATTCTCGGGTGCCAAGGAACTCC253650.12287228461454917RNA PCR Primer, Index 1 (100% over 27bp)
ACAGACCGGTAGACTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCA253520.12280931044936136RNA PCR Primer, Index 1 (100% over 28bp)
ACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCTGGAATTC253500.12279962211625553No Hit
ATGTAGGCAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA253270.12268820628553861RNA PCR Primer, Index 1 (100% over 28bp)
ACGGCGGTCCTTATGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC252370.12225223129577678RNA PCR Primer, Index 1 (100% over 31bp)
AGAGATCGGAAAGAACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT247250.11977201802068711RNA PCR Primer, Index 1 (100% over 30bp)
GTGCCGGCCGGGGGACGGACTGGGAACGGCTCTCTCGGGATGGAATTCT246910.1196073163578882No Hit
ATGGAACAATGTAGGCAAGGGAAGTCGGCATGGAATTCTCGGGTGCCAA243850.1181250013926979No Hit
ATAGGACTCCGCTGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCA236650.11463720147460305RNA PCR Primer, Index 1 (100% over 28bp)
AGTTTGGAACCCTGAACAGACTGCCGGTGATTGGAATTCTCGGGTGCCA233290.11300956151282546No Hit
AGACCGGTAGACTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT232820.11278188568483873RNA PCR Primer, Index 1 (100% over 30bp)
AATGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC232570.11266078152101597RNA PCR Primer, Index 1 (100% over 26bp)
GTTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGTGGAATTCTCGGG231950.11236044319473559No Hit
AAAGGTCGACGCGGGCTCTGCCCGTTTGGAATTCTCGGGTGCCAAGGAA229370.11111064822408494RNA PCR Primer, Index 1 (100% over 23bp)
TTGACCGAGATCTTTGGAGATGATTCTGTACTACTGGAATTCTCGGGTG225940.10944909909643699No Hit
GGCTGTCAGCGGACTGCTCGAGCTGCTTCCGCGGTGGAATTCTCGGGTG221440.10726922414762773No Hit
GCGGATATAGTCGAATGGTAAAATTTCTCCTTGGAATTCTCGGGTGCCA218560.10587410418038978No Hit
AGCCATCGTCACTGGTAAGGGACCGATAGAGAACCTTGCTGACCTGGAA218070.10563674001929722No Hit
TAGGACTCCGCTGGCACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT214720.10401394422407254RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)