FastQCFastQC Report
Sun 2 Jul 2023
SRR3749604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3749604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21577935
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAGGA4083091.892252432867186RNA PCR Primer, Index 1 (100% over 22bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAG3634051.6841509625457673No Hit
TCAGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGAACTCC3559301.6495090934327126RNA PCR Primer, Index 1 (100% over 27bp)
AGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2539631.1769569238205602RNA PCR Primer, Index 1 (100% over 31bp)
ACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG2025670.9387691639630947RNA PCR Primer, Index 1 (100% over 29bp)
TCAGACTGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAA1829850.848019052796294RNA PCR Primer, Index 1 (100% over 23bp)
AGACCGGTAGACTTGAACCTTGTTCCTACTGGAATTCTCGGGTGCCAAG1378670.6389258286300334No Hit
ACGAGGATCCATTGGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1314710.6092844380150372RNA PCR Primer, Index 1 (100% over 29bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTACTGGAATTCTCGGGTGCCA1199600.5559382767628135No Hit
ACAGACCGGTAGACTTGAACCTTGTTCCTACATGGAATTCTCGGGTGCC1058400.4905010604582876No Hit
TGACCGAGATCTTTGGAGATGATTCTGTACTTGGAATTCTCGGGTGCCA1024020.47456811784816294No Hit
ACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACTGGAA893380.4140247896751936No Hit
TCAGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCA863750.4002931698515173RNA PCR Primer, Index 1 (100% over 28bp)
AACAGACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAA796510.36913170792293143No Hit
TGACCGAGATCTTTGGAGATGATTCTGTACTACTGGAATTCTCGGGTGC771410.3574994548829626No Hit
TGACCGAGATCTTTGGAGATGTGGAATTCTCGGGTGCCAAGGAACTCCA746200.3458162238416234RNA PCR Primer, Index 1 (100% over 28bp)
AGACCGGTAGACTTGAACCTTGTTCCTACATGGAATTCTCGGGTGCCAA744110.3448476418155862No Hit
AGACTGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT696950.32299198231897536RNA PCR Primer, Index 1 (100% over 25bp)
AGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTGCCAAGGAACT655250.3036666854358399RNA PCR Primer, Index 1 (100% over 25bp)
TGACCGAGATCTTTGGAGATGATTCTGTGGAATTCTCGGGTGCCAAGGA608330.2819222506694918RNA PCR Primer, Index 1 (100% over 22bp)
ATGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG571300.26476120166271705RNA PCR Primer, Index 1 (100% over 29bp)
TGTTATTAATACTTGGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG535650.24823969485495254RNA PCR Primer, Index 1 (100% over 29bp)
TGACCGAGATCTTTGGAGATGATTCTGTACTGGAATTCTCGGGTGCCAA521090.2414920612190184No Hit
AATGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCA518650.24036127646134814RNA PCR Primer, Index 1 (100% over 28bp)
TTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGTGGAATTCTCGGGT501980.2326357920718549No Hit
ACGGGCTTGGCAGAATCAGCGGGGAATGGAATTCTCGGGTGCCAAGGAA485560.22502616677638523RNA PCR Primer, Index 1 (100% over 23bp)
AAGGCAACAAATTAGAGTCTCTGATTCACTTGGAATTCTCGGGTGCCAA481240.22302412163165752No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGGGTGCCAAG471670.21858903551243433No Hit
ATGGAACAATGTAGGCAAGGGAAGTCGGCATGGAATTCTCGGGTGCCAA461160.21371831919968245No Hit
TTATTAATACTTGGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC436120.20211387234227926RNA PCR Primer, Index 1 (100% over 31bp)
AATGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTC424290.19663142001308279RNA PCR Primer, Index 1 (100% over 26bp)
ACGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAAC407730.18895691362495995RNA PCR Primer, Index 1 (100% over 24bp)
AAAGGTCGACGCGGGCTCTGCCCGTTGCTGGAATTCTCGGGTGCCAAGG399700.1852355195249221Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGCTATGGTGAAATTGGTAGACACGCTGCTCTGGAATTCTCGGGTGC399290.1850455106107234No Hit
AGTTGGGGGCTCGAAGACGATCTGGAATTCTCGGGTGCCAAGGAACTCC399080.1849481889717436RNA PCR Primer, Index 1 (100% over 27bp)
TTCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGAA367270.1702062778481815RNA PCR Primer, Index 1 (100% over 23bp)
ATAGGACTCCGCTGGCACCTTGGAATTCTCGGGTGCCAAGGAACTCCAG355070.1645523540598301RNA PCR Primer, Index 1 (100% over 29bp)
AGCCGCGGTATCTTTTTCACTCTGGAATTCTCGGGTGCCAAGGAACTCC351860.16306472329256716RNA PCR Primer, Index 1 (100% over 27bp)
ACGGCGGTCCTTATGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC343910.15938040410261686RNA PCR Primer, Index 1 (100% over 31bp)
ACTGGATAACTAGCACTGAAAACCGTCTTGGAATTCTCGGGTGCCAAGG343220.15906063300311174Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGACCGGTAGACTTGAACCTTGTTCCTGGAATTCTCGGGTGCCAAGG339560.15736445586660633Illumina Small RNA Adapter 2 (100% over 21bp)
ACACGGACCAAGGAGTCTGACATGTGTGGAATTCTCGGGTGCCAAGGAA335920.1556775474576228RNA PCR Primer, Index 1 (100% over 23bp)
ATAGGACTCCGCTGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCA335850.1556451069112962RNA PCR Primer, Index 1 (100% over 28bp)
ACAACGGCGGTCCTTATGAACTGGAATTCTCGGGTGCCAAGGAACTCCA333310.15446797851601649RNA PCR Primer, Index 1 (100% over 28bp)
AGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC333050.15434748505823193RNA PCR Primer, Index 1 (100% over 27bp)
AGGGACGCATTTATTAGATATGGAATTCTCGGGTGCCAAGGAACTCCAG331140.15346232157989168RNA PCR Primer, Index 1 (100% over 29bp)
AGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTGGAATTCTCGGGTGCC328060.152034937541521No Hit
ATGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCC327840.15193298153878024RNA PCR Primer, Index 1 (100% over 27bp)
AACGAGGATCCATTGGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA321300.14890210763912304RNA PCR Primer, Index 1 (100% over 28bp)
ATGTAGGCAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA299890.13897993482694243RNA PCR Primer, Index 1 (100% over 28bp)
AGTAAACCCGTAAGGCGCAAGGAAGCTGTGGAATTCTCGGGTGCCAAGG295930.1371447267776087Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGACCGGTAGACTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCA293800.13615760729652768RNA PCR Primer, Index 1 (100% over 28bp)
AGAGATCGGAAAGAACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT289840.13432239924719394RNA PCR Primer, Index 1 (100% over 30bp)
TGAGTTTGGAACCCTGAACAGACTGCCGGTGATTGGAATTCTCGGGTGC287930.1334372357688537No Hit
TTGGCGGCGGAGAAAGACTCGGCATGGAATTCTCGGGTGCCAAGGAACT286840.13293209011891083RNA PCR Primer, Index 1 (100% over 25bp)
TTGACCGAGATCTTTGGAGATGATTCTGTACTACTGGAATTCTCGGGTG283660.13145836244293072No Hit
TTCCGAACCCGTCGGCTGTCAGCGGACTGGAATTCTCGGGTGCCAAGGA278150.1289048280106507RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGATGATGGAAAAACCAGCTGGAATTCTCGGGTGCCAAGGAACTCC276830.1282930919942061RNA PCR Primer, Index 1 (100% over 27bp)
AAGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAACTC271650.12589249156603724RNA PCR Primer, Index 1 (100% over 26bp)
AGCATCGGCTAACTCTGTGCCAGCTGGAATTCTCGGGTGCCAAGGAACT270100.12517416518309096RNA PCR Primer, Index 1 (100% over 25bp)
TTGTGGATTGGATGATGGAAAAACCAGCTGGAATTCTCGGGTGCCAAGG264090.12238891256276378Illumina Small RNA Adapter 2 (100% over 21bp)
TTGACCGAGATCTTTGGAGATGATTCTGTGGAATTCTCGGGTGCCAAGG262200.12151301781194539Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGTGCGCTGGATTATGACTGTGGAATTCTCGGGTGCCAAGGAACTCC262200.12151301781194539RNA PCR Primer, Index 1 (100% over 27bp)
ATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTGGAATTCTCGGGT261700.1212812996238982No Hit
TTTGGCATATGCCTGCTTTGACCGAGATCTTTGGAGATGTGGAATTCTC250100.11590543766120344No Hit
ACCGGTAGACTTGAACCTTGTTCCTTGGAATTCTCGGGTGCCAAGGAAC247690.11478855599481601RNA PCR Primer, Index 1 (100% over 24bp)
ATGCGGACTACCTGCACCTGGACTGGAATTCTCGGGTGCCAAGGAACTC245830.11392656433528046RNA PCR Primer, Index 1 (100% over 26bp)
AGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTATGGAATTCTCGGGTG243420.112809682668893No Hit
AGTAAGGATTGACAGACTGAGAGCTCTGGAATTCTCGGGTGCCAAGGAA241860.11208672192218579RNA PCR Primer, Index 1 (100% over 23bp)
AACACGGACCAAGGAGTCTGACATGTGTGGAATTCTCGGGTGCCAAGGA232080.10755431416398278RNA PCR Primer, Index 1 (100% over 22bp)
TGACTTGTAGGGAGGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT231870.10745699252500296RNA PCR Primer, Index 1 (100% over 30bp)
TTGACCGAGATCTTTGGAGATGATTCTGTACTTGGAATTCTCGGGTGCC231130.10711404960669314No Hit
AACGGGCTTGGCAGAATCAGCGGGGATGGAATTCTCGGGTGCCAAGGAA230810.10696574996634292RNA PCR Primer, Index 1 (100% over 23bp)
AACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCA230760.10694257814753821RNA PCR Primer, Index 1 (100% over 28bp)
AGCGGACTGCTCGAGCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG230230.1066969568682082RNA PCR Primer, Index 1 (100% over 29bp)
ATGAGAACTTTGAAGGCCGAAGAGGGGTGGAATTCTCGGGTGCCAAGGA228530.10590911502884774RNA PCR Primer, Index 1 (100% over 22bp)
AGTTTGGAACCCTGAACAGACTGCCGGTGATTGGAATTCTCGGGTGCCA227730.10553836592797226No Hit
ACGGGCCAAGGGACAGTCTCAGGTAGACAGTTGGAATTCTCGGGTGCCA222580.10315166859108621No Hit
GTTCCGAACCCGTCGGCTGTCAGCGGATGGAATTCTCGGGTGCCAAGGA217080.10060276852256717RNA PCR Primer, Index 1 (100% over 22bp)
AGACCGGTAGACTTGAACCTTGTTCCTGGAATTCTCGGGTGCCAAGGAA216920.10052861870239205RNA PCR Primer, Index 1 (100% over 23bp)
AGGCATCCTAACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAA216370.10027372869554013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)