FastQCFastQC Report
Mon 25 Sept 2023
SRR387872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20915375
Total Bases836.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA2434291.1638758568756238No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA2186491.045398421018031No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA1730100.8271905237176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA995210.47582699330038314No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA851130.4069398707888336No Hit
GGCGCCAGTGAAATACCACTACCTTTAAAAAAAAAAAAAA741830.3546816636087089No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAA587770.28102293169498516No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA458440.21918803750829233No Hit
GGCGCCAGTGAAATACCACTACCTTTATAAAAAAAAAAAA435540.2082391542107182No Hit
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA418950.200307190284659No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA356950.1706639254615325No Hit
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAAAAAA355310.16987981329524335No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA346950.16588275371586692No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAA339390.16226818787614375No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAAAAAA329390.15748701613047816No Hit
GGAATGCAGCTCTAAGTGGGTGGTAAAAAAAAAAAAAAAA277630.1327396711749132No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277190.1325292996181039No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA271070.12960322250975656No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAA265940.12715048140423013No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA262900.12569700519354782No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA259060.12386103524321224No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAAAAAAAAAAAA218160.10430604280344007No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217380.10393311140727815No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)