FastQCFastQC Report
Mon 25 Sept 2023
SRR387873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR387873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18479865
Total Bases739.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA5115412.7680992258330894No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAA4618122.4990009396713666No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA3824992.0698149039508675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2608121.4113306563657255No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAA1866441.009985733120886No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAA1572380.8508611940617531No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAA1320980.7148212392244208No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAA1298360.7025808900660259No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAA986420.5337809556509205No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAA926250.5012211939859951No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA894480.4840295099558357No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAA778840.4214532952486395No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC765920.41446190218380924No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAA731900.39605267679174067No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC730600.39534920844930416No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAA640310.3464906264196194No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAA625670.33856849062479627No Hit
ATAAATCATTTGTATACGACTTAGATAAAAAAAAAAAAAA619150.3350403263227302No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC608390.32921777296533283No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA602170.32585194751152136No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAA556470.301122329627408No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAA535920.2900021185219697No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC534720.2893527631289515No Hit
TGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC514440.2783786569869423No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAA472450.25565662952624385No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA466160.25225292500783963No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA462400.2502182781097156No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT450990.24404399058110005No Hit
TGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAA440890.23857858268986273No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC433730.23470409551152024No Hit
GTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT432370.23396815939943283No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAA429530.2324313516359562No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAA414950.22454168361078397No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAA414770.22444428030183122No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT412720.2233349648387583No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAA401400.21720937896461903No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAAAAAA399050.21593772465329156No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAA391570.21189007603681087No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC391470.21183596308739266No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC389130.21056972007100702No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAA380230.20575366757278798No Hit
TTGATGATTCATAATAACTTTTCGAATCACAAAAAAAAAA357270.19332933438637134No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAA347960.18829141879553773No Hit
TGTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAA345350.18687907081572294No Hit
GGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC338300.18306410788174046No Hit
TGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT338110.1829612932778459No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAA329270.17817770854927784No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT308610.16699797319947954Illumina Paired End PCR Primer 2 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG303940.16447089846165003No Hit
TTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT302680.1637890752989808No Hit
CGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC298110.1613161135105695No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290350.15711694863571785No Hit
GTAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT287710.1556883667710776Illumina Paired End PCR Primer 2 (95% over 21bp)
TTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAA285630.15456281742317923No Hit
GTTAATAGATAATATACGGATCTTAATAAAAAAAAAAAAA278000.15043399938257124No Hit
CTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC273350.14791774723462536No Hit
CCATATATATAATTTTAATTATATATAAAAAAAAAAAAAA269860.1460292052999305No Hit
GCAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG269490.1458289873870832No Hit
GGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAA268150.1451038738648794No Hit
ATATAAAGAATGGTACAGCAATTAATATAAAAAAAAAAAA260680.14106163654334056No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259080.14019582935264951No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA247130.13372933189717565No Hit
AGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT246260.13325854923723737No Hit
TTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAAA241190.1305150227017351No Hit
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG240030.1298873124884841Illumina PCR Primer Index 2 (95% over 22bp)
CGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT236930.12820981105652018No Hit
TGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT231990.12553663135526152Illumina Paired End PCR Primer 2 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT230750.12486563078247595No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAA228660.12373467013963575No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAA228130.12344787150771934No Hit
GGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA227820.12328012136452295No Hit
ATTTTAAAACCTAAAGGTAAACCTTTATAAAAAAAAAAAA222730.12052577223913702No Hit
TGTACAACGGGGTATTGTAAGCAAAAAAAAAAAAAAAAAA221060.11962208598385324No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA216710.11726817268416193No Hit
CCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGC216110.11694349498765277No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAA214970.1163266073642854No Hit
GTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG206680.11184064385751735No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT206080.11151596616100821No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT205580.11124540141391726No Hit
AATACTGAATATCTAAATATTATTATTACAAAAAAAAAAA202040.1093298030045133No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAA201480.10902677048777142No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAA199830.10813390682237127No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAA195230.10564471114913448No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTTG195220.10563929985419267Illumina PCR Primer Index 1 (95% over 22bp)
ATTTTAAAACCTAAAGGTAAACCTTTAAAAAAAAAAAAAA192620.10423236316931968No Hit
CTAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCT192310.10406461302612328No Hit
AGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192280.10404837914129783No Hit
TTAAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG192190.10399967748682146No Hit
AAATAGATAATATACGGATCTTAATAAAAAAAAAAAAAAA192120.10396179842222873No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT191960.10387521770315962Illumina Paired End PCR Primer 2 (95% over 21bp)
CGAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTGCTT191100.10340984633816319Illumina PCR Primer Index 1 (95% over 21bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATCAAAAAAAAAA185220.10022800491237353No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)